The RIB element in the goaG-pspF intergenic region of Escherichia coli

The sequence (2,700 bp) between the aldH and pspF genes of Escherichia coli was determined, The pspF gene encodes a sigma(54) transcriptional activator of the phage shock protein (psp) operon (pspA to pspE), Downstream of the pspF transcribed region are two open reading frames (ORFs), ordL, and goaG, convergently oriented with respect to pspF, These two ORFs, together with the adjacent aldH gene, may constitute a novel operon (aldH-ordL-goaG), The goaG-pspF intergenic region contains a complex extragenic mosaic element, RIB, The structure of this RIB element, which belongs to the BIME-1 family, is Y(REP1) > 16 < Z(1)(REP2), where Y and Z(1) are palindromic units and the central 16 bases contain an L motif with an ihf consensus sequence. DNA fragments containing the L motif of the psp RIB element effectively bind integration host factor (IHF), while the Y palindromic unit (REP1) of the same RIB element binds DNA gyrase weakly. Computer prediction of the pspF mRNA secondary structure suggested that the transcribed stem-loop structures formed by the 3'-flanking region of the pspF transcript containing the RIB element can stabilize and protect pspF mRNA, Analysis of pspF steady-state mRNA levels showed that transcripts,vith an intact RIB element are much more abundant than those truncated at the 3' end by deletion of either the entire RIB element or a single Z(1) sequence (REP2), Thus, the pspF 3'-flanking region containing the RIB element has an important role in the stabilization of the pspF transcript.