Bcl-2 and Bax proteins are increased in neocortical but not in thalamic apoptosis following devascularizing lesion of the cerebral cortex in the rat: an immunohistochemical study

被引:27
作者
Sulejczak, D [1 ]
Czarkowska-Bauch, J [1 ]
Macias, M [1 ]
Skup, M [1 ]
机构
[1] Polish Acad Sci, M Nencki Inst Expt Biol, Dept Neurophysiol, Lab Reinnervat Proc, 3 Pasteur St, PL-02093 Warsaw, Poland
关键词
cortical infarct; thalamus; death protein; neurodegeneration; neuroprotection;
D O I
10.1016/j.brainres.2004.01.063
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The hypothesis that devascularization of somatosensory and motor Cortex Causes apoptosis in infarctcd regions and in the linked thalamic nuclei was evaluated. To unravel whether Bcl-related proteins, known to regulate apoptosis, participate in neuronal and glial responses to devascularization, we analyzed immunohistochemically the distribution and intensity of staining of Bcl-2 and Bax proteins at different time points after lesion. Both early (up to 6 h) and late (1 -7 days) responses were studied. Devascularization led to rapid (within hours) apoptosis in the cortex and to a delayed (within 3 - 7 days) apoptosis in thalamic nuclei. In control groups, Bcl-2 and Bax immunoreactivity (IR) was detected in neurons and oligodendrocytes but not in astrocytes or microglia. Following devascularization, Bcl-2 IR and Bax IR increased in neurons before the onset of the apoptosis. In the ischemic focus, the increase reached maximal values 3 h after the lesion. The increase was of slower onset in the penumbra zone (24 h and after), a region in which both proteins were induced in astrocytes also. The change of Bax IR intensity exceeded four times that of Bcl-2 at all time points investigated, indicating a diminution of Bcl-2/Bax ratio that may direct neurons to apoptotic pathway. In numerous neurons, an increase of IR in the cytoplasm was accompanied by induction of nuclear staining. No changes of Bcl-2 and Bax IR were found in thalamic nuclei. Our results point to different mechanisms underlying apoptosis of cortical and thalamic neurons. Nuclear appearance of Bcl-2 and Bax suggests they possess regulatory role of gene expression changes triggered by cortical infarct. (C) 2004 Elsevier B.V All rights reserved.
引用
收藏
页码:133 / 149
页数:17
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