Monitoring the Interaction of a Single G-Protein Key Binding Site with Rhodopsin Disk Membranes upon Light Activation

被引：20

作者：

Kim, Tai-Yang ^{[1
]}

Uji-i, Hiroshi ^{[2
]}

Moeller, Martina ^{[1
]}

Muls, Benoit ^{[2
]}

Hofkens, Johan ^{[2
]}

Alexiev, Ulrike ^{[1
]}

机构：

[1] Free Univ Berlin, Dept Phys, D-14195 Berlin, Germany

[2] Katholieke Univ Leuven, Div Mol & Nano Mat, B-3001 Louvain, Belgium

来源：

BIOCHEMISTRY | 2009年 / 48卷 / 18期

关键词：

PARTICLE TRACKING; LATERAL DIFFUSION; COUPLED RECEPTORS; MICROSCOPY; MICRODOMAINS; SIMULATIONS; DYNAMICS; MOBILITY; REVEALS;

D O I：

10.1021/bi900308c

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Heterotrimeric G-proteins interact with their G-protein-coupled receptors (GPCRs) via key binding elements comprising the receptor-specific C-terminal segment of the alpha-subunit and the lipid anchors at the alpha-subunit N-terminus and the gamma-subunit C-terminus. Direct information about diffusion and interaction of GPCRs and their G-proteins is mandatory for all understanding of the signal transduction mechanism. By using single-particle tracking, we show that the encounters of the alpha-subunit C-terminus With the GPCR rhodopsin change after receptor activation. Slow as well as less restricted diffusion compared to the inactive state within domains 60-280 nm in length was found for the receptor-bound C-terminus, indicating short-range order in rhodopsin packing.

引用

页码：3801 / 3803

页数：3

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