Optimization of liquid media for lipase production by Candida rugosa

被引：58

作者：

Benjamin, S ^{[1
]}

Pandey, A ^{[1
]}

机构：

[1] CSIR,REG RES LAB,BIOTECHNOL UNIT,TRIVANDRUM 695019,KERALA,INDIA

来源：

BIORESOURCE TECHNOLOGY | 1996年 / 55卷 / 02期

关键词：

Candida rugosa; lipase; olive oil; submerged fermentation;

D O I：

10.1016/0960-8524(95)00194-8

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Studies were carried out on lipase production by Candida rugosa The yeast was grown in Universal Yeast medium. Addition of olive oil to this medium induced lipase production and in 10% (v/v) concentration yielded 9.36 U/ml enzyme. Replacement of glucose with maltose further improved the lipase yield. Maximum enzyme (12.55 U/ml) was obtained when C. rugosa was cultivated in Universal Yeast medium supplemented with ur ea (0.1%), maltose (1%), olive oil (10%, v/v) and minerals and incubated on a rotary shaker (180 rpm) at 30 +/- 1 degrees C for 72 h. Copyright (C) 1996 Elsevier Science Ltd.

引用

页码：167 / 170

页数：4

共 14 条

[1]

DELRIO JL, 1990, BIOTECHNOL LETT, V12, P335

[2]

FERRER P, 1992, APPL MICROBIOL BIOT, V37, P737

[3]

KAUR J, 1993, BIOTECHNOL LETT, V14, P257

[4]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[5] PRESENT AND FUTURE APPLICATIONS OF LIPASES [J].

MACRAE, AR ;

HAMMOND, RC .

BIOTECHNOLOGY & GENETIC ENGINEERING REVIEWS, 1985, 3 :193-217

[6] USE OF DINITROSALICYLIC ACID REAGENT FOR DETERMINATION OF REDUCING SUGAR [J].

MILLER, GL .

ANALYTICAL CHEMISTRY, 1959, 31 (03) :426-428

[7]

OBRADORS N, 1993, AGR BIOL CHEM TOKYO, V32, P357

[8] STEROL REQUIREMENT FOR LIPASE PRODUCTION BY CANDIDA CYLINDRACEA [J].

OTA, Y ;

MIYAIRI, S ;

YAMADA, K .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1968, 32 (12) :1476-&

[9] OPTIMIZING PRODUCTION OF EXTRACELLULAR LIPASE FROM RHODOTORULA-GLUTINIS [J].

PAPAPARASKEVAS, D ;

CHRISTAKOPOULOS, P ;

KEKOS, D ;

MACRIS, BJ .

BIOTECHNOLOGY LETTERS, 1992, 14 (05) :397-402

[10]

RAO PV, 1993, PROCESS BIOCHEM, V28, P391

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