Involvement of a Glu71-Arg64 couple in the access channel for NADH in cytochrome P450nor

被引：6

作者：

Su, F

Fushinobu, S

Takaya, N

Shoun, H ^{[1
]}

机构：

[1] Univ Tsukuba, Inst Appl Biochem, Tsukuba, Ibaraki 3058572, Japan

[2] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Biotechnol, Bunkyo Ku, Tokyo 1138657, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2004年 / 68卷 / 05期

关键词：

cytochrome P450nor; NADH binding; nitric oxide reductase; salt bridge network;

D O I：

10.1271/bbb.68.1156

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Putative access channel for NADH in the heme-distal pocket of cytochrome P450nor (P450nor) comprises many charged amino acid residues. Characterization of the E71A mutant protein of P450nor highlights the existence of a unique mechanism for binding NADH that depends on the salt bridge network between Glu71, Arg64 and Asp88.

引用

收藏

页码：1156 / 1159

页数：4

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