Protein folding;
Nascent chain;
Urea;
Ribosome;
ABP-120;
NMR;
FILAMINS;
ROD;
D O I:
10.1007/s12104-008-9134-4
中图分类号:
Q6 [生物物理学];
学科分类号:
071011 ;
摘要:
The gelation factor from Dictyostelium discoideum (ABP-120) is an actin binding protein consisting of six immunoglobulin (Ig) domains in the C-terminal rod domain. We have recently used the pair of domains 5 and 6 of ABP-120 as a model system for studying multi-domain nascent chain folding on the ribosome. Here we present the NMR assignments of domain 5 in its native and 8M urea-denatured states.