SARS corona virus peptides recognized by antibodies in the sera of convalescent cases

被引：99

作者：

Guo, JP

Petric, M

Campbell, W

McGeer, PL

机构：

[1] Univ British Columbia, Kinsmen Lab Neurol Res, Vancouver, BC V6T 1Z3, Canada

[2] BC Ctr Dis Conrol, Vancouver, BC, Canada

来源：

VIROLOGY | 2004年 / 324卷 / 02期

基金：

加拿大健康研究院;

关键词：

immune response; SARS vaccine; peptide membranes;

D O I：

10.1016/j.virol.2004.04.017

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We synthesized on cellulose membranes 4942 ten-amino-acid peptides which included all of the sequences predicted for the severe acute respiratory syndrome (SARS) corona virus. We probed these membranes with four pairs of acute and convalescent sera from recovered SARS cases. We correlated positively reacting peptides with the in vitro SARS-CoV neutralizing activity of the samples. We found that convalescent sera with high neutralizing activity recognized exclusively only a limited number of peptides on the membranes. This suggests that antibodies against the epitopes represented by these peptides could be responsible for much of the SARS-CoV neutralizing activity. The findings have implications for monitoring humoral responses to SARS-CoV as well as for developing a successful SARS vaccine. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：251 / 256

页数：6

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