Potential of on-line isotachophoresis-capillary zone electrophoresis with hydrodynamic counterflow in the analysis of various basic proteins and recombinant human interleukin-3

被引：32

作者：

Bergmann, J

Jaehde, U

Mazereeuw, M

Tjaden, UR

Schunack, W

机构：

[1] FREE UNIV BERLIN,INST PHARM,D-12169 BERLIN,GERMANY

[2] LEIDEN AMSTERDAM CTR DRUG RES,DIV ANALYT CHEM,2300 RA LEIDEN,NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 734卷 / 02期

关键词：

isotachophoresis capillary zone electrophoresis; proteins; interleukin-3;

D O I：

10.1016/0021-9673(95)01315-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Isotachophoresis-capillary zone electrophoresis with hydrodynamic counterflow was applied to the analysis of various basic model proteins and recombinant human interleukin-3. The use of coated capillaries and simple modifications of a commercial capillary zone electrophoresis (CZE) system allowed the detection of concentrations in the 10(-8) M region. The limit of detection was decreased by a factor of 100 compared to conventional CZE. The separation efficiency was unaffected by the preceding isotachophoretic step. Our method is applicable to the analysis of proteins with an isoelectric point higher than 7.0.

引用

页码：381 / 389

页数：9

共 30 条

[1] ISOTACHOPHORESIS SUPERIMPOSED ON CAPILLARY ZONE ELECTROPHORESIS [J].