An easy procedure to transform the ratio of two polynomials of first degree into Michaelis-Menten-type equations. Application to the ordered Uni Bi enzyme mechanism

被引：1

作者：

Fontes, R ^{[1
]}

Ribeiro, JM ^{[1
]}

Sillero, A ^{[1
]}

机构：

[1] Univ Autonoma Madrid, Fac Med, CSIC, Dept Bioquim,Inst Invest Biomed Alberto Sols, E-28029 Madrid, Spain

来源：

ACTA BIOCHIMICA POLONICA | 2000年 / 47卷 / 01期

关键词：

enzyme equations; enzyme inhibition; enzyme kinetics; graphical presentations; rectangular hyperbolas;

D O I：

10.18388/abp.2000_4083

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

It is not always clear that some equations affected by complicated factors can, actually, be interpreted as a ratio of two polynomials of first degree and so that they can be, in general, represented by rectangular hyperbolas. In this paper we present an easy procedure to rearrange those equations into Michaelis-Menten-type equations and so to make the aspects of these rectangular hyperbolas more clear, particularly for researchers familiar with general biochemistry. As an example, the method is applied to transform the classical rate equation of the Cleland's Ordered Uni Bi enzyme mechanism.

引用

页码：259 / 268

页数：10

共 7 条

[1]

KINETICS OF ENZYME-CATALYZED REACTIONS WITH 2 OR MORE SUBSTRATES OR PRODUCTS .1. NOMENCLATURE AND RATE EQUATIONS [J].