Antibody Responses to SARS-CoV-2 in Patients With Novel Coronavirus Disease 2019

被引:804
作者
Zhao, Juanjuan [1 ]
Yuan, Quan [2 ,3 ,4 ]
Wang, Haiyan [1 ]
Liu, Wei [2 ,3 ,4 ]
Liao, Xuejiao [1 ]
Su, Yingying [2 ,3 ,4 ]
Wang, Xin [1 ]
Yuan, Jing [5 ]
Li, Tingdong [2 ,3 ,4 ]
Li, Jinxiu [6 ]
Qian, Shen [1 ]
Hong, Congming [2 ,3 ,4 ]
Wang, Fuxiang [5 ]
Liu, Yingxia [5 ,7 ]
Wang, Zhaoqin [7 ]
He, Qing [7 ]
Li, Zhiyong [4 ]
He, Bin [2 ,3 ,4 ]
Zhang, Tianying [2 ,3 ,4 ]
Fu, Yang [8 ]
Ge, Shengxiang [2 ,3 ,4 ]
Liu, Lei [1 ,7 ]
Zhang, Jun [2 ,3 ,4 ]
Xia, Ningshao [2 ,3 ,4 ]
Zhang, Zheng [1 ,7 ]
机构
[1] Shenzhen Third Peoples Hosp, Natl Clin Res Ctr Infect Dis, Inst Hepatol, Shenzhen, Peoples R China
[2] Xiamen Univ, Collaborat Innovat Ctr Biol Prod, Sch Publ Hlth, Natl Inst Diagnost & Vaccine Dev Infect Dis,State, Xiamen, Peoples R China
[3] Xiamen Univ, Sch Life Sci, Xiamen, Peoples R China
[4] Xiamen Univ, Sch Publ Hlth, Xiamen, Peoples R China
[5] Shenzhen Third Peoples Hosp, Dept Infect Dis, Shenzhen, Peoples R China
[6] Shenzhen Third Peoples Hosp, Dept Crit Care Med, Shenzhen, Peoples R China
[7] Southern Univ Sci & Technol, Affiliated Hosp 2, Sch Med, Shenzhen, Peoples R China
[8] Southern Univ Sci & Technol, Sch Med, Shenzhen, Peoples R China
基金
比尔及梅琳达.盖茨基金会; 俄罗斯科学基金会; 美国国家科学基金会;
关键词
antibody; SARS-CoV-2; COVID-19; VIRAL LOAD;
D O I
10.1093/cid/ciaa344
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background. The novel coronavirus SARS-CoV-2 is a newly emerging virus. The antibody response in infected patients remains largely unknown, and the clinical value of antibody testing has not been fully demonstrated. Methods. 173 patients with SARS-CoV-2 infection were enrolled. Their serial plasma samples (n = 535) collected during hospitalization were tested for total antibodies (Ab), IgM, and IgG against SARS-CoV-2. The dynamics of antibodies with disease progress were analyzed. Results. Among 173 patients, the seroconversion rates for Ab, IgM, and IgG were 93.1%, 82.7%, and 64.7%, respectively. The reason for the negative antibody findings in 12 patients might be due to the lack of blood samples at the later stage of illness. The median seroconversion times for Ab, IgM, and then IgG were days 11, 12, and 4, respectively. The presence of antibodies was <40% among patients within 1 week of onset, and rapidly increased to 100.0% (Ab), 94.3% (IgM), and 79.8% (IgG) by day 15 after onset. In contrast, RNA detectability decreased from 66.7% (58/87) in samples collected before day 7 to 45.5% (25/55) during days 15-39. Combining RNA and antibody detection significantly improved the sensitivity of pathogenic diagnosis for COVID-19 (P < .001), even in the early phase of 1 week from onset (P = .007). Moreover, a higher titer of Ab was independently associated with a worse clinical classification (P = .006). Conclusions. Antibody detection offers vital clinical information during the course of SARS-CoV-2 infection. The findings provide strong empirical support for the routine application of serological testing in the diagnosis and management of COVID-19 patients.
引用
收藏
页码:2027 / 2034
页数:8
相关论文
共 10 条
[1]  
[Anonymous], 2020, CLIN DIAGNOSIS 8274, DOI 10.1101/2020.02.12.20022327
[2]   A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster [J].
Chan, Jasper Fuk-Woo ;
Yuan, Shuofeng ;
Kok, Kin-Hang ;
To, Kelvin Kai-Wang ;
Chu, Hin ;
Yang, Jin ;
Xing, Fanfan ;
Liu, Jieling ;
Yip, Cyril Chik-Yan ;
Poon, Rosana Wing-Shan ;
Tsoi, Hoi-Wah ;
Lo, Simon Kam-Fai ;
Chan, Kwok-Hung ;
Poon, Vincent Kwok-Man ;
Chan, Wan-Mui ;
Ip, Jonathan Daniel ;
Cai, Jian-Piao ;
Cheng, Vincent Chi-Chung ;
Chen, Honglin ;
Hui, Christopher Kim-Ming ;
Yuen, Kwok-Yung .
LANCET, 2020, 395 (10223) :514-523
[3]   Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR (Publication with Expression of Concern) [J].
Corman, Victor M. ;
Landt, Olfert ;
Kaiser, Marco ;
Molenkamp, Richard ;
Meijer, Adam ;
Chu, Daniel K. W. ;
Bleicker, Tobias ;
Bruenink, Sebastian ;
Schneider, Julia ;
Schmidt, Marie Luisa ;
Mulders, Daphne G. J. C. ;
Haagmans, Bart L. ;
van der Veer, Bas ;
van den Brink, Sharon ;
Wijsman, Lisa ;
Goderski, Gabriel ;
Romette, Jean-Louis ;
Ellis, Joanna ;
Zambon, Maria ;
Peiris, Malik ;
Goossens, Herman ;
Reusken, Chantal ;
Koopmans, Marion P. G. ;
Drosten, Christian .
EUROSURVEILLANCE, 2020, 25 (03) :23-30
[4]   Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China (vol 395, pg 497, 2020) [J].
Huang, C. ;
Wang, Y. ;
Li, X. .
LANCET, 2020, 395 (10223) :496-496
[5]  
Huang CL, 2020, LANCET, V395, P497, DOI [10.1016/S0140-6736(20)30211-7, 10.1016/S0140-6736(20)30183-5]
[6]   Anti-spike IgG causes severe acute lung injury by skewing macrophage responses during acute SARS-CoV infection [J].
Liu, Li ;
Wei, Qiang ;
Lin, Qingqing ;
Fang, Jun ;
Wang, Haibo ;
Kwok, Hauyee ;
Tang, Hangying ;
Nishiura, Kenji ;
Peng, Jie ;
Tan, Zhiwu ;
Wu, Tongjin ;
Cheung, Ka-Wai ;
Chan, Kwok-Hung ;
Alvarez, Xavier ;
Qin, Chuan ;
Lackner, Andrew ;
Perlman, Stanley ;
Yuen, Kwok-Yung ;
Chen, Zhiwei .
JCI INSIGHT, 2019, 4 (04)
[7]   Clinical progression and viral load in a community outbreak of coronavirus-associated SARS pneumonia: a prospective study [J].
Peiris, JSM ;
Chu, CM ;
Cheng, VCC ;
Chan, KS ;
Hung, IFN ;
Poon, LLM ;
Law, KI ;
Tang, BSF ;
Hon, TYW ;
Chan, CS ;
Chan, KH ;
Ng, JSC ;
Zheng, BJ ;
Ng, WL ;
Lai, RWM ;
Guan, Y ;
Yuen, KY .
LANCET, 2003, 361 (9371) :1767-1772
[8]  
Wan S., 2020, CHARACTERISTICS LYMP, DOI 10.1101/2020.02.10.20021832
[9]  
Yang Y, 2020, medRxiv, DOI [10.1101/2020.02.11.20021493, 10.1101/2020.02.11.20021493, 10.1101/2020.02.11.20021493, DOI 10.1101/2020.02.11.20021493]
[10]  
Zou LR, 2020, NEW ENGL J MED, V382, P1177, DOI [10.1148/radiol.2020200463, 10.1056/NEJMc2001737]