Pharmaceutical therapies for sealing of permeabilized cell membranes in electrical injuries

被引:33
作者
Lee, RC [1 ]
Hannig, J
Matthews, KL
机构
[1] Univ Chicago, Dept Surg, Chicago, IL 60637 USA
[2] Univ Chicago, Dept Radiol, Chicago, IL 60637 USA
来源
OCCUPATIONAL ELECTRICAL INJURY: AN INTERNATIONAL SYMPOSIUM | 1999年 / 888卷
关键词
D O I
10.1111/j.1749-6632.1999.tb07961.x
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
Several years ago, we proposed that loss of cell membrane structural integrity by electroporation is a substantial cause of tissue necrosis in victims of electrical trauma.(1) Specifically, this involves the permeabilization of the lipid bilayer by thermal and electrical forces. We further suggested that certain mild surfactants in low concentration could induce scaling of permeabilized lipid bilayers and salvage of cells that had not been extensively heat-damaged. Successful restoration of membrane transport properties using the surfactant poloxamer 188 was reported In 1992.(2) The purpose of this study is to further examine the response of electroporated rat skeletal muscle membranes to poloxamer 188 (P188) therapy by direct assay of membrane transport properties. Experimental evidence accumulated to date suggests that P188 is effective in sealing permeabilized cell membranes bath irt vitro and in vivo.
引用
收藏
页码:266 / 273
页数:8
相关论文
共 20 条
[1]   A MINIATURE GAMMA-CAMERA [J].
AARSVOLD, JN ;
MINTZER, RA ;
YASILLO, NJ ;
HEIMSATH, SJ ;
BLOCK, TA ;
MATTHEWS, KL ;
PAN, X ;
WU, C ;
BECK, RN ;
CHEN, CT ;
COOPER, M .
ELECTRICAL INJURY: A MULTIDISCIPLINARY APPROACH TO THERAPY, PREVENTION, AND REHABILITATION, 1994, 720 :192-205
[2]   The effects of ethylene oxide containing lipopolymers and tri-block copolymers on lipid bilayers of dipalmitoylphosphatidylcholine [J].
Baekmark, TR ;
Pedersen, S ;
Jorgensen, K ;
Mouritsen, OG .
BIOPHYSICAL JOURNAL, 1997, 73 (03) :1479-1491
[3]  
BHATT DL, 1990, PLAST RECONSTR SURG, V86, P1
[4]  
Block Thomas A., 1995, Journal of Burn Care and Rehabilitation, V16, P581, DOI 10.1097/00004630-199511000-00004
[5]   CHANGES IN MEMBRANE-STRUCTURE INDUCED BY ELECTROPORATION AS REVEALED BY RAPID-FREEZING ELECTRON-MICROSCOPY [J].
CHANG, DC ;
REESE, TS .
BIOPHYSICAL JOURNAL, 1990, 58 (01) :1-12
[6]  
Halliwell B., 1991, FREE RADICALS BIOL M
[7]   Poloxamine 1107 sealing of radiopermeabilized erythrocyte membranes [J].
Hannig, J ;
Yu, J ;
Beckett, M ;
Weichselbaum, R ;
Lee, RC .
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1999, 75 (03) :379-385
[8]   TRENDS IN EVOLUTION - IN THE BEGINNING [J].
HORGAN, J .
SCIENTIFIC AMERICAN, 1991, 264 (02) :116-125
[9]   MICELLE FORMATION AND SOLUBILIZATION OF FLUORESCENT-PROBES IN POLY(OXYETHYLENE-B-OXYPROPYLENE-B-OXYETHYLENE) SOLUTIONS [J].
KABANOV, AV ;
NAZAROVA, IR ;
ASTAFIEVA, IV ;
BATRAKOVA, EV ;
ALAKHOV, VY ;
YAROSLAVOV, AA ;
KABANOV, VA .
MACROMOLECULES, 1995, 28 (07) :2303-2314
[10]   NUCLEATION AND GROWTH OF ICE CRYSTALS INSIDE CULTURED-HEPATOCYTES DURING FREEZING IN THE PRESENCE OF DIMETHYL-SULFOXIDE [J].
KARLSSON, JOM ;
CRAVALHO, EG ;
RINKES, IHMB ;
TOMPKINS, RG ;
YARMUSH, ML ;
TONER, M .
BIOPHYSICAL JOURNAL, 1993, 65 (06) :2524-2536