The Role of GW182 Proteins in miRNA-Mediated Gene Silencing

被引：96

作者：

Braun, Joerg E. ^{[1
]}

Huntzinger, Eric ^{[1
]}

Izaurralde, Elisa ^{[1
]}

机构：

[1] Max Planck Inst Dev Biol, Dept Biochem, D-72076 Tubingen, Germany

来源：

TEN YEARS OF PROGRESS IN GW/P BODY RESEARCH | 2013年 / 768卷

关键词：

MESSENGER-RNA DEADENYLATION; TRANSLATIONAL REPRESSION; AIN-1; INTERACTS; REQUIRES GW182; ARGONAUTE; DOMAINS; IDENTIFICATION; TARGETS; BINDING; MOTIFS;

D O I：

10.1007/978-1-4614-5107-5_9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

GW182 family proteins are essential for microRNA-mediated gene silencing in animal cells. They are recruited to miRNA targets through direct interactions with Argonaute proteins and promote target silencing. They do so by repressing translation and enhancing mRNA turnover. Although the precise mechanism of action of GW182 proteins is not fully understood, these proteins have been shown to interact with the cytoplasmic poly(A)-binding protein (PABP) and with the PAN2-PAN3 and CCR4-NOT deadenylase complexes. These findings suggest that GW182 proteins function as scaffold proteins for the assembly of the multiprotein complex that silences miRNA targets.

引用

页码：147 / 163

页数：17

共 62 条

[1]

Functional Dissection of the Human TNRC6 (GW182-Related) Family of Proteins [J].

Baillat, David ;

Shiekhattar, Ramin .

MOLECULAR AND CELLULAR BIOLOGY, 2009, 29 (15) :4144-4155

[2]

MicroRNAs: Target Recognition and Regulatory Functions [J].

Bartel, David P. .

CELL, 2009, 136 (02) :215-233

[3]

Ribosome Profiling Shows That miR-430 Reduces Translation Before Causing mRNA Decay in Zebrafish [J].

Bazzini, Ariel A. ;

Lee, Miler T. ;

Giraldez, Antonio J. .

SCIENCE, 2012, 336 (6078) :233-237

[4]

MicroRNAs silence gene expression by repressing protein expression and/or by promoting mRNA decay [J].

Behm-Ansmant, I. ;

Rehwinkel, J. ;

Izaurralde, E. .

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 2006, 71 :523-530

[5]

Kinetic analysis reveals successive steps leading to miRNA-mediated silencing in mammalian cells [J].

Bethune, Julien ;

Artus-Revel, Caroline G. ;

Filipowicz, Witold .

EMBO REPORTS, 2012, 13 (08) :716-723

[6]

RNA-directed DNA methylation requires an AGO4-interacting member of the SPT5 elongation factor family [J].

Bies-Etheve, Natacha ;

Pontier, Dominique ;

Lahmy, Sylvie ;

Picart, Claire ;

Vega, Danielle ;

Cooke, Richard ;

Lagrange, Thierry .

EMBO REPORTS, 2009, 10 (06) :649-654

[7]

GW182 Proteins Directly Recruit Cytoplasmic Deadenylase Complexes to miRNA Targets [J].

Braun, Joerg E. ;

Huntzinger, Eric ;

Fauser, Maria ;

Izaurralde, Elisa .

MOLECULAR CELL, 2011, 44 (01) :120-133

[8]

From UBA to UBX: new words in the ubiquitin vocabulary [J].

Buchberger, A .

TRENDS IN CELL BIOLOGY, 2002, 12 (05) :216-221

[9]

miRNA repression involves GW182-mediated recruitment of CCR4-NOT through conserved W-containing motifs [J].

Chekulaeva, Marina ;

Mathys, Hansruedi ;

Zipprich, Jakob T. ;

Attig, Jan ;

Colic, Marija ;

Parker, Roy ;

Filipowicz, Witold .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2011, 18 (11) :1218-U62

[10]

The GW/WG repeats of Drosophila GW182 function as effector motifs for miRNA-mediated repression [J].

Chekulaeva, Marina ;

Parker, Roy ;

Filipowicz, Witold .

NUCLEIC ACIDS RESEARCH, 2010, 38 (19) :6673-6683

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