An improved clearing method for GUS assay in Arabidopsis endosperm and seeds

被引：58

作者：

Stangeland, B ^{[1
]}

Salehian, Z ^{[1
]}

机构：

[1] Agr Univ Norway, Dept Chem & Biotechnol, As, Norway

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 2002年 / 20卷 / 02期

关键词：

Arabidopsis; chloral hydrate; clearing; endosperm; GUS; Hoyer's medium; lactophenol; seed;

D O I：

10.1007/BF02799426

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Precise cellular localization of the GUS stain is notoriously difficult in Arabidopsis seeds. Here we report an improved protocol for the clearing of seeds after GUS staining. Incubation in ethanol-acetic acid (EtAc) and Hoyer's medium allows reliable cellular localization of the GUS, even in seeds from late developmental stages. This method also leads to the staining of nucleoli in the endosperm and embryo, facilitating nuclear counts in endosperm development.

引用

页码：107 / 114

页数：8

共 14 条

[1]

Beeckman T., 1994, Plant Molecular Biology Reporter, V12, P37, DOI 10.1007/BF02668662

[2]

BERLETH T, 1993, DEVELOPMENT, V118, P575

[3] Dynamic analyses of the expression of the HISTONE::YFP fusion protein in arabidopsis show that syncytial endosperm is divided in mitotic domains [J].