Detection of harmful cyanobacteria and their toxins by both PCR amplification and LC-MS during a bloom event

被引：46

作者：

dos Anjos, Fabyana Maria

Bittencourt-Oliveira, Maria do Carmo

Zajac, Meron Petro

Hiller, Susann

Christian, Bernd

Erler, Katrin

Luckas, Bernd

Pinto, Ernani ^{[1
]}

机构：

[1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, BR-05508900 Sao Paulo, Brazil

[2] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Ciencias Biol, Piracicaba, SP, Brazil

[3] CETESB, Companhia Tecnol Saneamento Ambiental, Sao Paulo, Brazil

[4] Univ Jena, Fac Biol & Pharm, Dept Food Chem, D-07743 Jena, Germany

来源：

TOXICON | 2006年 / 48卷 / 03期

基金：

巴西圣保罗研究基金会;

关键词：

microcystis; microcystin; saxitoxin; neosaxitoxin; gonyautoxin; mycB; harmful algae bloom; liquid chromatography-mass spectrometry; PCR; Billings reservoir;

D O I：

10.1016/j.toxicon.2006.05.006

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We briefly report here the occurrence of toxic blooms in the eutrophic reservoir Billings, Sao Paulo city, Brazil. Water samples were collected in May 2004, during a cyanobacterial bloom. The presence of toxic species was confirmed by using PCR amplifications of a fragment region of genes encoding microcystin synthetase-mcyB. The determination of toxins was performed by liquid chromatography coupled with mass spectrometry (LC-MS). LC-MS analyses of the toxins from the bloom revealed variants of microcystins (MC), such as MC-LR, MC-RR and MC-YR. HPLC-FLD was used to determine the paralytic shellfish poisoning (PSP) saxitoxin (STX), neosaxitoxin (NEO), gonyautoxins 2 (GTX2) and 3 (GTX3). GTX2, GTX3 and NEO were detected for the first time in a natural sample from Billings reservoir. These results are a contribution to the knowledge of the biogeography of toxic cyanobacteria and their toxins, specifically in Sao Paulo. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：239 / 245

页数：7

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