Antisenescence effect of mouse embryonic stem cell conditioned medium through a PDGF/FGF pathway

被引：31

作者：

Bae, Yun-Ui ^{[1
,2
]}

Choi, Joon-Hyuk ^{[3
]}

Nagy, Andras ^{[4
]}

Sung, Hoon-Ki ^{[4
,5
]}

Kim, Jae-Ryong ^{[1
,2
]}

机构：

[1] Yeungnam Univ, Coll Med, Dept Biochem & Mol Biol, 170 Hyunchung Ro, Daegu 705717, South Korea

[2] Yeungnam Univ, Coll Med, Smart Aging Convergence Res Ctr, Daegu 705717, South Korea

[3] Yeungnam Univ, Coll Med, Dept Pathol, Daegu 705717, South Korea

[4] Mt Sinai Hosp, Lunenfeld Tanenbaum Res Inst, Toronto, ON M5G 1X5, Canada

[5] Hosp Sick Children, Res Inst, Physiol & Expt Med Program, Toronto, ON M5G 0A4, Canada

来源：

FASEB JOURNAL | 2016年 / 30卷 / 03期

基金：

新加坡国家研究基金会;

关键词：

cellular senescence; conditioned medium; embryonic stem cells; PDGF-BB; PLASMINOGEN-ACTIVATOR INHIBITOR-1; GROWTH-FACTOR BB; REPLICATIVE SENESCENCE; LIFE-SPAN; ENDOTHELIAL-CELLS; PROGENITOR CELLS; IN-VITRO; ANGIOGENESIS; MICE; FIBROBLASTS;

D O I：

10.1096/fj.15-278846

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Cellular senescence, an irreversible state of growth arrest, underlies organismal aging and age-related diseases. Recent evidence suggests that aging intervention based on inhibition of cellular senescence might be a promising strategy for treatment of aging and age-related diseases. Embryonic stem cells (ESCs) and ESC conditioned medium (CM) have been suggested as a desirable source for regenerative medicine. However, effects of ESC-CM on cellular senescence remain to be determined. We found that treatment of senescent human dermal fibroblasts with CM from mouse ESCs (mESCs) decreases senescence phenotypes. We found that platelet-derived growth factor BB in mESC-CM plays a critical role in antisenescence effect of mESC-CM through up-regulation of fibroblast growth factor 2. We confirmed that mESC-CM treatment accelerates the wound-healing process by down-regulating senescence-associated p53 expression in in vivo models. Taken together, our results suggest that mESC-CM has the ability to suppress cellular senescence and maintain proliferative capacity. Therefore, this strategy might emerge as a novel therapeutic strategy for aging and age-related diseases.

引用

页码：1276 / 1286

页数：11

共 49 条

[1]

EGF promotes mammalian cell growth by suppressing cellular senescence [J].

Alexander, Peter B. ;

Yuan, Lifeng ;

Yang, Pengyuan ;

Sun, Tao ;

Chen, Rui ;

Xiang, Handan ;

Chen, Jiekai ;

Wu, Haoyu ;

Radiloff, Daniel R. ;

Wang, Xiao-Fan .

CELL RESEARCH, 2015, 25 (01) :135-138

[2]

Angiogenic synergism, vascular stability and improvement of hind-limb ischemia by a combination of PDGF-BB and FGF-2 [J].

Cao, RH ;

Bråkenhielm, E ;

Pawliuk, R ;

Wariaro, D ;

Post, MJ ;

Wahlberg, E ;

Leboulch, P ;

Cao, YH .

NATURE MEDICINE, 2003, 9 (05) :604-613

[3]

R Regulation of tumor angiogenesis and metastasis by FGF and PDGF signaling pathways [J].

Cao, Yihai ;

Cao, Renhai ;

Hedlund, Eva-Maria .

JOURNAL OF MOLECULAR MEDICINE-JMM, 2008, 86 (07) :785-789

[4]

PDGF signalling controls age-dependent proliferation in pancreatic β-cells [J].

Chen, Hainan ;

Gu, Xueying ;

Liu, Yinghua ;

Wang, Jing ;

Wirt, Stacey E. ;

Bottino, Rita ;

Schorle, Hubert ;

Sage, Julien ;

Kim, Seung K. .

NATURE, 2011, 478 (7369) :349-+

[5]

DDS, 4,4′-diaminodiphenylsulfone, extends organismic lifespan [J].

Cho, Sung Chun ;

Park, Moon Cheol ;

Keam, Bhumsuk ;

Choi, Jung Min ;

Cho, Yunje ;

Hyun, Soonsil ;

Park, Sang Chul ;

Lee, Junho .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (45) :19326-19331

[6]

Restoration of senescent human diploid fibroblasts by modulation of the extracellular matrix [J].

Choi, Hae Ri ;

Cho, Kyung A. ;

Kang, Hyun Tae ;

Lee, Jung Bin ;

Kaeberlein, Matt ;

Suh, Yousin ;

Chung, In Kwon ;

Park, Sang Chul .

AGING CELL, 2011, 10 (01) :148-157

[7]

Rejuvenation of aged progenitor cells by exposure to a young systemic environment [J].

Conboy, IM ;

Conboy, MJ ;

Wagers, AJ ;

Girma, ER ;

Weissman, IL ;

Rando, TA .

NATURE, 2005, 433 (7027) :760-764

[8]

Embryonic anti-aging niche [J].

Conboy, Irina M. ;

Yousef, Hanadie ;

Conboy, Michael J. .

AGING-US, 2011, 3 (05) :555-563

[9]

Novel Cell-Free Strategy for Therapeutic Angiogenesis: In Vitro Generated Conditioned Medium Can Replace Progenitor Cell Transplantation [J].

Di Santo, Stefano ;

Yang, Zijiang ;

von Ballmoos, Moritz Wyler ;

Voelzmann, Jan ;

Diehm, Nicolas ;

Baumgartner, Iris ;

Kalka, Christoph .

PLOS ONE, 2009, 4 (05)

[10]

A BIOMARKER THAT IDENTIFIES SENESCENT HUMAN-CELLS IN CULTURE AND IN AGING SKIN IN-VIVO [J].

DIMRI, GP ;

LEE, XH ;

BASILE, G ;

ACOSTA, M ;

SCOTT, C ;

ROSKELLEY, C ;

MEDRANO, EE ;

LINSKENS, M ;

RUBELJ, I ;

PEREIRASMITH, O ;

PEACOCKE, M ;

CAMPISI, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (20) :9363-9367

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