Quantification of a cytochrome P450 3A4 substrate, buspirone, in human plasma by liquid chromatography-tandem mass spectrometry

被引：8

作者：

Chew, Wade M. ^{[1
]}

Xu, Min-Jian ^{[1
]}

Cordova, Catherine A. ^{[1
]}

Chow, H-H. Sherry ^{[1
]}

机构：

[1] Univ Arizona, Arizona Canc Ctr, Tucson, AZ 85724 USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2006年 / 844卷 / 02期

关键词：

buspirone; human plasma; liquid chromatography; tandem mass spectrometry; electrospray ionization;

D O I：

10.1016/j.jchromb.2006.07.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A sensitive HPLC-tandem mass spectrometry method was developed for determination of buspirone levels in human plasma. After solid phase extraction and reversed phase HPLC separation, detection of buspirone and the internal standard (prazosin) was performed using eletrospray ionization and selected reaction monitoring in the positive ion mode. Linear calibration curves were established over a concentration range of 0.025-2.5 ng/ml when 0.5 ml aliquots of plasma were used. Satisfactory results of within-day precision (RSD of 1.9-7.7%) and accuracy (% difference of 0.5-6.6%) and between-day precision (RSD of 3.7-11.1%) and accuracy (% difference of 2.2-6.8%) were obtained. The assay has been successfully applied to the analysis of buspirone levels in more than 500 human plasma samples collected from a drug interaction study. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：235 / 239

页数：5

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