A novel fluorometric method for DNA and RNA determination

被引：69

作者：

Li, WY ^{[1
]}

Xu, JG ^{[1
]}

Guo, XQ ^{[1
]}

Zhu, QZ ^{[1
]}

Zhao, YB ^{[1
]}

机构：

[1] XIAMEN UNIV,DEPT CHEM,RES LAB,SEDC ANALYT SCI MAT & LIFE CHEM,XIAMEN 361005,PEOPLES R CHINA

来源：

ANALYTICAL LETTERS | 1997年 / 30卷 / 03期

基金：

中国国家自然科学基金;

关键词：

DNA; RNA; methylene blue; fluorescence quenching;

D O I：

10.1080/00032719708001799

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for the determination of nucleic acids with methylene blue(MB) as a fluorescence probe was developed, based on the quenching of the fluorescence of MB in the presence of nucleic acids. The calibration graph was linear in the range of 0-40 mu g/ml for CT DNA and 0-55 mu g/ml for yeast RNA, the limit of detection was 28 ng/ml for CT DNA and 82 ng/ml for yeast RNA. CT DNA could be determined in the presence of 30% (w/w) yeast RNA and yeast RNA was determined when the content of CT DNA in synthetic samples was below 5% (w/w).

引用

页码：527 / 536

页数：10

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