Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation

被引：49

作者：

Elkord, Eyad ^{[1
]}

机构：

[1] Univ Manchester, Dept Med Oncol, Clin Immunotherapy Lab, Manchester M20 4BX, Lancs, England

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2009年 / 347卷 / 1-2期

关键词：

T regulatory cell; Cryopreservation; INCREASED POPULATIONS; CARCINOMA; FOXP3;

D O I：

10.1016/j.jim.2009.06.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cryopreservation of peripheral blood mononuclear cells (PBMC) is essential for many clinical and research assays. Some studies reported consistent changes in PBMC phenotype following cryopreservation. We hypothesized that PBMC freezing may have a negative impact on estimation of the frequency of T regulatory cell (Treg). Treg levels were measured in 6 fresh PBMC samples isolated from 6 healthy donors and these levels were re-measured after freezing for three weeks. Herein, we report a significant reduction in Treg frequency in all samples following cryopreservation. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：87 / 90

页数：4

共 11 条

[1] Human CD4+ CD25+ regulatory T cells control T-cell responses to human immunodeficiency virus and cytomegalovirus antigens
Aandahl, EM
Michaëlsson, J
Moretto, WJ
Hecht, FA
Nixon, DF
[J]. JOURNAL OF VIROLOGY, 2004, 78 (05) : 2454 - 2459
[2] Bignone PA, 2008, EXPERT OPIN BIOL TH, V8, P1897, DOI [10.1517/14712590802494022, 10.1517/14712590802494022 ]
[3] Effects of cryopreservation on lymphocyte immunophenotype and function
Costantini, A
Mancinia, S
Giuliodoro, S
Butini, L
Regnery, CM
Silvestri, G
Montroni, M
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 2003, 278 (1-2) : 145 - 155
[4] Ex vivo isolation and characterization of CD4+CD25+ T cells with regulatory properties from human blood
Dieckmann, D
Plottner, H
Berchtold, S
Berger, T
Schuler, G
[J]. JOURNAL OF EXPERIMENTAL MEDICINE, 2001, 193 (11) : 1303 - 1310
[5] Foxp3 Programs the Development and Function of CD4+CD25+ Regulatory T Cells (Reprinted from vol 4, pg 330-336, 2003)
Fontenot, Jason D.
Gavin, Marc A.
Rudensky, Alexander Y.
[J]. JOURNAL OF IMMUNOLOGY, 2017, 198 (03) : 986 - 992
[6] Frequency of regulatory T cells in renal cell carcinoma patients and investigation of correlation with survival
Griffiths, Richard W.
Elkord, Eyad
Gilham, David E.
Ramani, Vijay
Clarke, Noel
Stern, Peter L.
Hawkins, Robert E.
[J]. CANCER IMMUNOLOGY IMMUNOTHERAPY, 2007, 56 (11) : 1743 - 1753
[7] Ichihara F, 2003, CLIN CANCER RES, V9, P4404
[8] Prevalence of regulatory T cells is increased in peripheral blood and tumor microenvironment of patients with pancreas or breast adenocarcinoma
Liyanage, UK
Moore, TT
Joo, HG
Tanaka, Y
Herrmann, V
Doherty, G
Drebin, JA
Strasberg, SM
Eberlein, TJ
Goedegebuure, PS
Linehan, DC
[J]. JOURNAL OF IMMUNOLOGY, 2002, 169 (05) : 2756 - 2761
[9] Increased populations of regulatory T cells in peripheral blood of patients with hepatocellular carcinoma
Ormandy, LA
Hillemann, T
Wedemeyer, H
Manns, MP
Greten, TF
Korangy, F
[J]. CANCER RESEARCH, 2005, 65 (06) : 2457 - 2464
[10] Wolf AM, 2003, CLIN CANCER RES, V9, P606

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