Live-imaging of single stem cells within their niche reveals that a U3snoRNP component segregates asymmetrically and is required for self-renewal in Drosophila

被引：66

作者：

Fichelson, Pierre ^{[1
,2
,4
,5
]}

Moch, Clara ^{[1
,3
]}

Ivanovitch, Kenzo ^{[1
,2
,4
,5
]}

Martin, Charlotte ^{[3
]}

Sidor, Clara M. ^{[1
,2
,6
]}

Lepesant, Jean-Antoine ^{[1
,2
]}

Bellaiche, Yohanns ^{[3
]}

Huynh, Jean-Rene ^{[1
,3
]}

机构：

[1] Univ Paris 07, CNRS, Inst Jacques Monod, F-75251 Paris 05, France

[2] Univ Paris 06, CNRS, Inst Jacques Monod, F-75251 Paris 05, France

[3] Inst Curie, UMR 144, F-75248 Paris 05, France

[4] UCL, MRC, Mol Cell Biol Lab, London WC1E 6BT, England

[5] UCL, Dept Anat & Dev Biol, Cell Biol Unit, London WC1E 6BT, England

[6] London Res Inst, Canc Res UK, London WC2A 3PX, England

来源：

NATURE CELL BIOLOGY | 2009年 / 11卷 / 06期

基金：

英国医学研究理事会;

关键词：

OOCYTE SPECIFICATION; GERMLINE CYST; GENE; OVARY; DIVISION; TRANSCRIPTION; PROTEIN; PROLIFERATION; MELANOGASTER; MECHANISMS;

D O I：

10.1038/ncb1874

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Stem cells generate self-renewing and differentiating progeny over many rounds of asymmetric divisions. How stem cell growth rate and size are maintained over time remains unknown. We isolated mutations in a Drosophila melanogaster gene, wicked (wcd), which induce premature differentiation of germline stem cells (GSCs). Wcd is a member of the U3 snoRNP complex required for pre-ribosomal RNA maturation. This general function of Wcd contrasts with its specific requirement for GSC self-renewal. However, live imaging of GSCs within their niche revealed a pool of Wcd-forming particles that segregate asymmetrically into the GSCs on mitosis, independently of the Dpp signal sent by the niche. A fraction of Wcd also segregated asymmetrically in dividing larval neural stem cells (NSCs). In the absence of Wcd, NSCs became smaller and produced fewer neurons. Our results show that regulation of ribosome synthesis is a crucial parameter for stem cell maintenance and function.

引用

页码：685 / U11

页数：19

共 53 条

[1] Self-renewal and solid tumor stem cells [J].

Al-Hajj, M ;

Clarke, MF .

ONCOGENE, 2004, 23 (43) :7274-7282

[2] Frizzled regulates localization of cell-fate determinants and mitotic spindle rotation during asymmetric cell division [J].

Bellaïche, Y ;

Gho, M ;

Kaltschmidt, JA ;

Brand, AH ;

Schweisguth, F .

NATURE CELL BIOLOGY, 2001, 3 (01) :50-57

[3] The brain tumor gene negatively regulates neural progenitor cell proliferation in the larval central brain of Drosophila [J].

Bello, Bruno ;

Reichert, Heinrich ;

Hirth, Frank .

DEVELOPMENT, 2006, 133 (14) :2639-2648

[4] Asymmetric segregation of the tumor suppressor brat regulates self-renewal in Drosophila neural stem cells [J].

Betschinger, J ;

Mechtler, K ;

Knoblich, JA .

CELL, 2006, 124 (06) :1241-1253

[5] Dare to be different:: Asymmetric cell division in Drosophila, C-elegans and vertebrates [J].

Betschinger, J ;

Knoblich, JA .

CURRENT BIOLOGY, 2004, 14 (16) :R674-R685

[6] Slicing across kingdoms: Regeneration in plants and animals [J].

Birnbaum, Kenneth D. ;

Alvarado, Alejandro Sanchez .

CELL, 2008, 132 (04) :697-710

[7] The multifunctional nucleolus [J].

Boisvert, Francois-Michel ;

van Koningsbruggen, Silvana ;

Navascues, Joaquin ;

Lamond, Angus I. .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2007, 8 (07) :574-585

[8] Genetic dissection of a stem cell niche:: The case of the Drosophila ovary [J].

Bolivar, J. ;

Pearson, J. ;

Lopez-Onieva, L. ;

Gonzalez-Reyes, A. .

DEVELOPMENTAL DYNAMICS, 2006, 235 (11) :2969-2979

[9] The tumor suppressors brat and numb regulate transit-amplifying neuroblast lineages in Drosophila [J].

Bowman, Sarah K. ;

Rolland, Vivien ;

Betschinger, Joerg ;

Kinsey, Kaolin A. ;

Emery, Gregory ;

Knoblich, Juergen A. .

DEVELOPMENTAL CELL, 2008, 14 (04) :535-546

[10] Germline stem cell number in the Drosophila ovary is regulated by redundant mechanisms that control Dpp signaling [J].

Casanueva, MO ;

Ferguson, EL .

DEVELOPMENT, 2004, 131 (09) :1881-1890

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