Rapid isocratic HPLC analysis of beta-carotene in red peppers (Capsicum annuum L.) and food preparations

被引：15

作者：

Weissenberg, M

Levy, A

Schaeffler, I

Menagem, E

Barzilai, M

机构：

[1] Lab. of Natural Products Chemistry, Department of Plant Genetics, Volcani Center

来源：

CHROMATOGRAPHIA | 1997年 / 46卷 / 7-8期

关键词：

column liquid chromatography; beta-carotene; capsanthin and capsorubin; red peppers;

D O I：

10.1007/BF02490877

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, rapid high-performance liquid chromatography method has been devised in order to separate and quantify beta-carotene present in red pepper (Capsicum annuum L.) fruits and food preparations. A reversed-phase isocratic non-aqueous system enables the separation of beta-carotene within a few minutes, with detection at 450 nm. The selection of extraction solvents, mild saponification conditions, and chromatographic features, is evaluated and discussed. The method is proposed for rapid screening of large plant populations, plant selection, as well as for food products, and also for nutrition and quality control studies.

引用

页码：399 / 403

页数：5

共 30 条

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[Anonymous], CAROTENOIDS

[2] CAROTENOIDS AND THE IMMUNE-RESPONSE [J].