The impact of manuring on nitrogen isotope ratios in cereals: archaeological implications for reconstruction of diet and crop management practices

Recent archaeological studies of human diet have used stable nitrogen isotope ratios (delta N-15) from human bone collagen to infer the relative importance of terrestrial plant and animal foods. This approach is based on widely observed enrichment of delta N-15 up the food chain, plants having distinctly lower values than the herbivores that consume them. Studies of early farming diets in Britain, Denmark and Germany have tended to detect relatively high delta N-15 values (e.g. c. +9 parts per thousand), interpreted as evidence of a diet largely based on animal products, though arch acobotanical evidence for crop cultivation (e.g. carbonised cereal grain and chaff) is widespread. This paper investigates the impact of manuring on delta N-15 values in modern cereals, and of charring on these cereal values. The results from two long-term experiments demonstrate that manuring significantly raises delta N-15 in cereal grain and chaff. Depending on manuring levels and frequency, it appears that human diets with a major component of such grain would conventionally be interpreted as indicating a largely animal-based diet or a mixed plant/animal diet. Moreover, preliminary analyses of experimentally charred grain and chaff from manured and unmanured conditions are promising for the extraction of reliable ancient delta N-15 values from archaeobotanical cereal remains. The wider implications of these results, and the need for further work, are discussed. (c) 2006 Elsevier Ltd. All rights reserved.