A mild chemically cleavable linker system for functional proteomic applications

被引：131

作者：

Verhelst, Steven H. L.

Fonovic, Marko

Bogyo, Matthew

机构：

[1] Stanford Univ, Sch Med, Dept Pathol, Stanford, CA 94305 USA

[2] Stanford Univ, Sch Med, Dept Microbiol & Immunol, Stanford, CA 94305 USA

[3] Josef Stefan Inst, Dept Biochem & Mol Biol, Ljubljana 1000, Slovenia

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 08期

关键词：

activity-based probes; affinity purification; cleavable linkers; mass spectrometry; proteomics;

D O I：

10.1002/anie.200603811

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) An amicable split: Small-molecule probes can be applied for the enrichment of specific protein targets from a complex proteome. A cleavable linker system, which prevents the release of unwanted, nonspecifically bound proteins, can be used for a very mild and highly selective cleavage of probe-labeled proteins. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：1284 / 1286

页数：3

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