The complex relationship between soluble and insoluble tau in tauopathies revealed by efficient dephosphorylation and specific antibodies

被引:48
作者
Hanger, DP
Gibb, GM
de Silva, R
Boutajangout, A
Brion, JP
Revesz, T
Lees, AJ
Anderton, BH
机构
[1] Inst Psychiat, KCL, Dept Neurosci, London SE5 8AF, England
[2] UCL Royal Free & Univ Coll, Sch Med, Reta Lila Weston Inst Neurol Studies, London W1T 4JF, England
[3] Free Univ Brussels, Sch Med, Lab Histol & Neuropatholl, B-1070 Brussels, Belgium
[4] Inst Neurol, Dept Mol Neuropathol, London WC1N 1PJ, England
基金
英国医学研究理事会; 英国惠康基金;
关键词
tau; Alzheimer's disease; tauopathy; neurodegeneration; paired helical filament; lambda protein phosphatase;
D O I
10.1016/S0014-5793(02)03611-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Phosphorylated tau is deposited as insoluble inclusion bodies in the tauopathies. We have used a new efficient method to dephosphorylate tau extracted from control and tauopathy brain. In some tauopathies, including Alzheimer's disease and progressive supranuclear palsy, the pattern of insoluble tau isoforms reflected that of soluble tau. In contrast, in corticobasal degeneration, Pick's disease, and some forms of fronto-temporal dementia, specific tau isoforms were selectively sequestered into insoluble inclusion-forming tau. Therefore the overall expression of individual tau isoforms does not predict which tau isoforms are deposited in all tauopathies and different mechanisms must operate that result in the deposition of specific tau isoforms. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:538 / 542
页数:5
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