Harpin induces disease resistance in Arabidopsis through the systemic acquired resistance pathway mediated by salicylic acid and the NIM1 gene

被引：216

作者：

Dong, HS ^{[1
]}

Delaney, TP ^{[1
]}

Bauer, DW ^{[1
]}

Beer, SV ^{[1
]}

机构：

[1] Cornell Univ, Dept Plant Pathol, Ithaca, NY 14853 USA

来源：

PLANT JOURNAL | 1999年 / 20卷 / 02期

关键词：

D O I：

10.1046/j.1365-313x.1999.00595.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Harpin, the product of the hrpN gene of Erwinia amylovora, elicits the hypersensitive response and disease resistance in many plants. Harpin and known inducers of systemic acquired resistance (SAR) were tested on five genotypes of Arabidopsis thaliana to assess the role of SAR in harpin-induced resistance. In wild-type plants, harpin elicited systemic resistance to Peronospora parasitica and Pseudomonas syringae pv. tomato, accompanied by induction of the SAR genes PR-1 and PR-2. However, in experiments with transgenic Arabidopsis plants containing the nahG gene which prevents accumulation of salicylic acid (SA), harpin neither elicited resistance nor activated SAR gene expression. Harpin also failed to activate SAR when applied to nim1 (non-inducible immunity) mutants, which are defective in responding to SA and regulation of SAR. In contrast, mutants compromised in responsiveness to methyl jasmonate and ethylene developed the same resistance as did wild-type plants. Thus, harpin elicits disease resistance through the NIM1-mediated SAR signal transduction pathway in an SA-dependent fashion. The site of action of harpin in the SAR regulatory pathway is upstream of SA.

引用

页码：207 / 215

页数：9

共 74 条

[1] Rapid and transient activation of a myelin basic protein kinase in tobacco leaves treated with harpin from Erwinia amylovora [J].

Adam, AL ;

Pike, S ;

Hoyos, ME ;

Stone, JM ;

Walker, JC ;

Novacky, A .

PLANT PHYSIOLOGY, 1997, 115 (02) :853-861

[2] INCREASED TOLERANCE TO 2 OOMYCETE PATHOGENS IN TRANSGENIC TOBACCO EXPRESSING PATHOGENESIS-RELATED PROTEIN-1A [J].

ALEXANDER, D ;

GOODMAN, RM ;

GUTRELLA, M ;

GLASCOCK, C ;

WEYMANN, K ;

FRIEDRICH, L ;

MADDOX, D ;

AHLGOY, P ;

LUNTZ, T ;

WARD, E ;

RYALS, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (15) :7327-7331

[3] Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity [J].

Alvarez, ME ;

Pennell, RI ;

Meijer, PJ ;

Ishikawa, A ;

Dixon, RA ;

Lamb, C .

CELL, 1998, 92 (06) :773-784

[4]

[Anonymous], 1992, METHODS ARABIDOPSIS

[5] POPA1, A PROTEIN WHICH INDUCES A HYPERSENSITIVITY-LIKE RESPONSE ON SPECIFIC PETUNIA GENOTYPES, IS SECRETED VIA THE HRP PATHWAY OF PSEUDOMONAS-SOLANACEARUM [J].

ARLAT, M ;

VANGIJSEGEM, F ;

HUET, JC ;

PERNOLLET, JC ;

BOUCHER, CA .

EMBO JOURNAL, 1994, 13 (03) :543-553

[6] HARPIN, AN ELICITOR OF THE HYPERSENSITIVE RESPONSE IN TOBACCO CAUSED BY ERWINIA-AMYLOVORA, ELICITS ACTIVE OXYGEN PRODUCTION IN SUSPENSION CELLS [J].

BAKER, CJ ;

ORLANDI, EW ;

MOCK, NM .

PLANT PHYSIOLOGY, 1993, 102 (04) :1341-1344

[7]

Bauer D. W., 1997, Phytopathology, V87, pS7

[8] ERWINIA-CHRYSANTHEMI HARPIN(ECH) - AN ELICITOR OF THE HYPERSENSITIVE RESPONSE THAT CONTRIBUTES TO SOFT-ROT PATHOGENESIS [J].

BAUER, DW ;

WEI, ZM ;

BEER, SV ;

COLLMER, A .

MOLECULAR PLANT-MICROBE INTERACTIONS, 1995, 8 (04) :484-491

[9]

Beer S. V., 1991, Advances in molecular genetics of plant-microbe interactions. Vol. 1. Proceedings of the 5th international symposium on the molecular genetics of plant-microbe interactions, Interlaken, Switzerland, September 9-14, 1990., P53

[10] Pathogenesis-related functions of plant beta-1,3-glucanases investigated by antisense transformation - A review [J].

Beffa, R ;

Meins, F .

GENE, 1996, 179 (01) :97-103

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