Identification of Escherichia coli O157:H7- strains from pigs with postweaning diarrhoea and amplification of their virulence marker genes by PCR

被引:13
作者
Osek, J [1 ]
机构
[1] Natl Vet Res Inst, Dept Microbiol, PL-24100 Pulawy, Poland
关键词
D O I
10.1136/vr.150.22.689
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Escherichia coli isolated from pigs with postweaning diarrhoea were examined by PCR for the presence of the O157 rfb gene responsible for the biosynthesis of E coli O157 lipopolysaccharide. Among the 372 isolates tested, 38 (10.2 per cent) were of the O157 serogroup, but none of these possessed the H7 determinant Further analysis of the E coli O157 isolates revealed that seven of them had the genes responsible for the production of Shiga toxin I and eaeA intimin, four other strains had genes responsible for the production of Shiga toxin 2, and four other strains were positive for the enterohaemolysin gene.
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页码:689 / 692
页数:4
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