Triggering the TCR complex causes the downregulation of nonengaged receptors by a signal transduction-dependent mechanism

被引:100
作者
José, ES
Borroto, A
Niedergang, F
Alcover, A
Alarcón, B
机构
[1] Univ Autonoma Madrid, Ctr Biol Mol Severo Ochoa, E-28049 Madrid, Spain
[2] Inst Pasteur, Unite Biol Interact Cellulaires, F-75724 Paris 15, France
关键词
D O I
10.1016/S1074-7613(00)80169-7
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Downregulation of the TCR complex is believed to be intimately tied to T cell activation, allowing serial triggering of receptors and desensitization of stimulated cells. We studied transfected and transgenic T cells expressing CD3 xi chimeras to demonstrate that ligand engagement of the TCR or chimeras causes comodulation of nonengaged receptors. Comodulation required protein tyrosine kinase activity but not trans-phosphorylation of nonengaged receptors. The TCR appears to be downregulated by at least two mechanisms. One mechanism requires direct engagement, independent of signaling. The second requires signaling and downregulates nontriggered receptors. These results shed new light on the process of TCR downregulation and indicate that the number of downregulated TCRs cannot be assumed to equal the number of engaged receptors.
引用
收藏
页码:161 / 170
页数:10
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