Hyperoxia impairs postnatal alveolar epithelial development via NADPH oxidase in newborn mice

被引:31
作者
Auten, Richard L. [1 ]
Mason, S. Nicholas [1 ]
Auten, Kathryn M. [1 ]
Brahmajothi, Mulugu [1 ]
机构
[1] Duke Univ, Med Ctr, Dept Pediat, Neonatal Perinatal Res Inst,Div Neonatol, Durham, NC 27710 USA
关键词
bronchopulmonary dysplasia; reactive oxygen species; neutrophil cytosolic factor-1; p47(phox); INDUCED LUNG INFLAMMATION; BABOON MODEL; RAT LUNG; BRONCHOPULMONARY DYSPLASIA; NEUTROPHIL INFLUX; NEONATAL-RAT; DISEASE; INJURY; OXYGEN; CELLS;
D O I
10.1152/ajplung.00112.2009
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Auten RL, Mason SN, Auten KM, Brahmajothi M. Hyperoxia impairs postnatal alveolar epithelial development via NADPH oxidase in newborn mice. Am J Physiol Lung Cell Mol Physiol 297: L134-L142, 2009. First published May 1, 2009; doi: 10.1152/ajplung.00112.2009. Hyperoxia disrupts postnatal lung development in part through inducing inflammation. To determine the contribution of leukocyte-derived reactive oxygen species, we exposed newborn wild-type and NADPH oxidase p47(phox) subunit null (p47(phox-/-)) mice to air or acute hyperoxia (95% O(2)) for up to 11 days. Hyperoxia-induced pulmonary neutrophil influx was similar in wild-type and p47(-/-) mice at postnatal days (P) 7 and 11. Macrophages were decreased in wild-type hyperoxia-exposed mice compared with p47(phox-/-) mice at P11. Hyperoxia impaired type II alveolar epithelial cell and bronchiolar epithelial cell proliferation, but depression of type II cell proliferation was significantly less in p47(-/-) mice at P3 and P7, when inflammation was minimal. We found reciprocal results for the expression of the cell cycle inhibitor p21(cip/waf) in type II cells, which was induced in 95% O(2)-exposed wild-type mice, but significantly less in p47(phox-/-) littermates at P7. Despite partial preservation of type II cell proliferation, deletion of p47(phox) did not prevent the major adverse effects of hyperoxia on alveolar development estimated by morphometry at P11, but hyperoxia impairment of elastin deposition at alveolar septal crests was significantly worse in wild-type vs. p47(phox-/-) mice at P11. Since we found that p47(phox) is expressed in a subset of alveolar epithelial cells, its deletion may protect postnatal type II alveolar epithelial proliferation from hyperoxia through effects on epithelial as well as phagocyte-generated superoxide.
引用
收藏
页码:L134 / L142
页数:9
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