Formation of DNA-adducts by selected sex steroids in rat liver

1 We are reporting investigations into the potential of the steroid hormones chlormadinone acetate (CMA), cyproterone acetate (CPA), ethinylestradiol (EE(2)) gestodene (GEST), megestrol acetate (MGA), norethisterone acetate (NET-Ac), estradiol (E(2)), and progesterone (P) to form DNA-adducts in rat liver in vivo, 2 Compound-related DNA-adduct spots were detected in male and female rat liver following CMA, CPA, and MGA using the P-32-postlabeling-technique. Substance-specific DNA-adducts were also observed in male rats after administration of E(2). The other compounds showed no DNA-adduct formation. After treatment with CMA, CPA or MGA, the relative adduct labeling(RAL) differed sex- and substance-specifically.