Decreased osteopontin expression in the rat kidney on a sodium deficient diet

被引:4
作者
Cha, JH
Ahn, YH
Lim, SW
Kim, YH
Han, KH
Jung, JY
Kim, J
机构
[1] Catholic Univ Korea, Coll Med, Dept Anat, Seoul 137701, South Korea
[2] Yonsei Univ, Wonju Coll Med, Dept Nursing, Seoul 120749, South Korea
关键词
osteopontin; kidney; sodium-restricted diet; immunohistochemistry; in situ hybridization; Sprague-Dawley rats;
D O I
10.3349/ymj.2000.41.1.128
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Osteopontin (OPN) is a secreted phosphoprotein that is constitutively expressed in the normal kidney and is induced by various experimental and pathologic conditions. Several possible functions of OPN have been suggested, however the mechanism and significance of OPN expression are still uncertain. Since high sale concentration or salt crystal have been known to enhance OPN expression in intact kidney or cultured renal cells, in me present study we examined whether or not a low salt condition had an effect on OPN expression in the kidney. Adult male Sprague-Dawley rats were fed either a normal sodium or a sodium deficient dec for 1 week. Kidneys were processed for in situ hybridization using a digoxigenin-labeled riboprobe and for immunohistochemistry using antibodies to OPN, renin, and Na-K-ATPase. In rats fed a normal sodium diet, OPN mRNA and protein were expressed only in the descending thin limbs of Henle's loop (DTL) and in the papillary and pelvic surface epithelium (PSE). In rats fed a sodium deficient diet, there was a marked decrease in OPN immunoreactivity in the DTL, bur no changes in PSE. In contrast, no changes were observed in OPN mRNA expression in the DTL by in situ hybridization, indicating chat decreased OPN protein expression was a result of translational regulation. As expected, rats fed a sodium deficient diet were associated with increased immunoreactivity for Na-K-ATPase and renin compatible with activation of the renin-angiotensin system. These results suggest that dietary sodium may be involved in the regulation of OPN expression in the DTL of the rat kidney.
引用
收藏
页码:128 / 135
页数:8
相关论文
共 35 条
[1]   EXPRESSION AND DISTRIBUTION OF OSTEOPONTIN IN HUMAN TISSUES - WIDESPREAD ASSOCIATION WITH LUMINAL EPITHELIAL SURFACES [J].
BROWN, LF ;
BERSE, B ;
VANDEWATER, L ;
PAPADOPOULOSSERGIOU, A ;
PERRUZZI, CA ;
MANSEAU, EJ ;
DVORAK, HF ;
SENGER, DR .
MOLECULAR BIOLOGY OF THE CELL, 1992, 3 (10) :1169-1180
[2]   OSTEOPONTIN - A PROTEIN WITH DIVERSE FUNCTIONS [J].
DENHARDT, DT ;
GUO, XJ .
FASEB JOURNAL, 1993, 7 (15) :1475-1482
[3]  
DIAMOND JR, 1995, AM J PATHOL, V146, P1455
[4]   CALCIUM AND KIDNEY [J].
EPSTEIN, FH .
AMERICAN JOURNAL OF MEDICINE, 1968, 45 (05) :700-+
[5]   OSTEOPONTIN EXPRESSION IN ANGIOTENSIN-II-INDUCED TUBULOINTERSTITIAL NEPHRITIS [J].
GIACHELLI, CM ;
PICHLER, R ;
LOMBARDI, D ;
DENHARDT, DT ;
ALPERS, CE ;
SCHWARTZ, SM ;
JOHNSON, RJ .
KIDNEY INTERNATIONAL, 1994, 45 (02) :515-524
[6]  
Gokhale JA, 1996, NEPHRON, V73, P456
[7]   CALCIUM-OXALATE MONOHYDRATE CRYSTALS STIMULATE GENE-EXPRESSION IN RENAL EPITHELIAL-CELLS [J].
HAMMES, MS ;
LIESKE, JC ;
PAWAR, S ;
SPARGO, BH ;
TOBACK, FG .
KIDNEY INTERNATIONAL, 1995, 48 (02) :501-509
[8]  
HWANG S, 1994, J BIOL CHEM, V269, P711
[9]   AGE AND DEVELOPMENT-RELATED CHANGES IN OSTEOPONTIN AND NITRIC-OXIDE SYNTHASE MESSENGER-RNA LEVELS IN HUMAN KIDNEY PROXIMAL TUBULE EPITHELIAL-CELLS - CONTRASTING RESPONSES TO HYPOXIA AND REOXYGENATION [J].
HWANG, SM ;
WILSON, PD ;
LASKIN, JD ;
DENHARDT, DT .
JOURNAL OF CELLULAR PHYSIOLOGY, 1994, 160 (01) :61-68
[10]   Renal injury and salt-sensitive hypertension after exposure to catecholamines [J].
Johnson, RJ ;
Gordon, KL ;
Suga, S ;
Duijvestijn, AM ;
Griffin, K ;
Bidani, A .
HYPERTENSION, 1999, 34 (01) :151-159