Development of an immwofluorometric assay and quantification of human kallikrein 7 in tissue extracts and biological fluids

Background: Human kallikrein 7 (hK7), also known as human stratum corneum chymotryptic enzyme, is a chymotrypsin-like serine protease first identified in human skin extracts and predicted to be a secreted protease. The aim of this study was to develop a sensitive and specific immunoassay for hK7 and to examine the distribution of hK7 in tissue extracts and biological fluids. Methods: Recombinant hK7 was produced in human embryonic kidney cells (HEK293T) and purified by a three-step column chromatographic procedure. The purified hK7 was injected into mice for antibody generation. A sandwich-type immunoassay was developed with the anti-hK7 monoclonal antibodies. Results: The assay had imprecision (CV) <10% through the dynamic range of 0.2-20 mug/L and had no detectable cross-reacivity from other members in the human kallikrein gene family. Highest concentrations were found in skin, esophagus, and kidney. hK7 was also found in amniotic fluid, ascites from ovarian cancer. patients, breast milk, cerebrospinal fluid, saliva, seminal plasma, serum, sweat, synovial fluid, and urine. Conclusions: This study describes the first ELISA-type immunoassay for hK7 protein, quantification. hK7 is found many human tissues and in various biological fluids. (C) 2004 American Association for Clinical Chemistry.