Immortalization of normal adult human middle ear epithelial cells using a retrovirus containing the E6/E7 genes of human papillomavirus type 16

被引：75

作者：

Chun, YM

Moon, SK

Lee, HY

Webster, P

Brackmann, DE

Rhim, JS

Lim, DJ

机构：

[1] House Ear Res Inst, Gonda Dept Cell & Mol Biol, Los Angeles, CA 90057 USA

[2] House Ear Res Inst, Ahmanson Ctr Adv Electron Microscopy & Imaging, Los Angeles, CA 90057 USA

[3] House Ear Clin, Los Angeles, CA USA

[4] Uniformed Serv Univ Hlth Sci, Ctr Prostate Dis Res, Bethesda, MD USA

来源：

ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY | 2002年 / 111卷 / 06期

关键词：

human middle ear epithelial cell; human papillomavirus type 16; immortalization;

D O I：

10.1177/000348940211100606

中图分类号：

R76 [耳鼻咽喉科学];

学科分类号：

100213 [耳鼻咽喉科学];

摘要：

A human middle ear epithelial cell line (HMEEC-1) was established using human papillomavirus E6/E7 genes. HMEEC-1 has remained morphologically and phenotypically stable, even after 50 passages. The cells are anchorage-dependent and nontumorigenic when injected into nude mice. This cell line thus provides a new tool for the study of normal cell biology and the pathological processes associated with the epithelial cells of the middle ear in otitis media. HMEEC-1 will also be useful in the search for new drugs and biological agents for the treatment of otitis media.

引用

收藏

页码：507 / 517

页数：11

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