Cross-talk between posttranscriptionally silenced neomycin phosphotransferase II transgenes

被引：24

作者：

Van Houdt, H

Kovarík, A

Van Montagu, M

Depicker, A

机构：

[1] State Univ Ghent VIB, Dept Mol Genet, B-9000 Ghent, Belgium

[2] State Univ Ghent VIB, Dept Plant Genet, B-9000 Ghent, Belgium

[3] Acad Sci Czech Republ, Inst Biophys, CS-61265 Brno, Czech Republic

来源：

FEBS LETTERS | 2000年 / 467卷 / 01期

关键词：

cytosine methylation; gene silencing; inverted repeat; posttranscriptional gene silencing; T-DNA; tobacco;

D O I：

10.1016/S0014-5793(00)01076-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tobacco plants containing a transgene locus with two chimeric neomycin phosphotransferase II (nptII) genes in tail-to-tail orientation (locus 1) show posttranscriptional gene silencing. The silenced nptII transgenes of locus 1 can downregulate the expression of homologous nptII transgenes in hybrid plants. The 3' region of the silenced nptII genes located in the center of the inverted repeat locus 1 is extensively methylated. Moreover, 3' segments of in trans-inactivated transgenes also become methylated, suggesting cross-talk between homologous posttranscriptionally silenced genes. Our results are in accordance with the hypothesis that this cross-talk can be mediated by specially featured RNAs. (C) 2000 Federation of European Biochemical Societies.

引用

页码：41 / 46

页数：6

共 38 条

[1] METHYLATION INDUCED PREMEIOTICALLY IN ASCOBOLUS - COEXTENSION WITH DNA REPEAT LENGTHS AND EFFECT ON TRANSCRIPT ELONGATION [J].