Methyl Jasmonate Reduces Grain Yield by Mediating Stress Signals to Alter Spikelet Development in Rice

被引:202
作者
Kim, Eun Hye [1 ]
Kim, Youn Shic [1 ]
Park, Su-Hyun [1 ]
Koo, Yeon Jong [2 ]
Do Choi, Yang [2 ]
Chung, Yong-Yoon [3 ]
Lee, In-Jung [4 ]
Kim, Ju-Kon [1 ]
机构
[1] Myongji Univ, Sch Biotechnol & Environm Engn, Yongin 449728, South Korea
[2] Seoul Natl Univ, Sch Agr Biotechnol, Seoul 151921, South Korea
[3] Korea Univ, Sch Life Sci & Biotechnol, Seoul 136701, South Korea
[4] Kyungpook Natl Univ, Sch Appl Biosci, Taegu 702701, South Korea
关键词
ABSCISIC-ACID; WATER-STRESS; UBIQUITIN PROMOTER; GENE-EXPRESSION; SALT-TOLERANT; HIGH-LEVEL; ARABIDOPSIS; GROWTH; RESPONSES; ETHYLENE;
D O I
10.1104/pp.108.134684
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Jasmonic acid (JA) is involved in plant development and the defense response. Transgenic overexpression of the Arabidopsis (Arabidopsis thaliana) jasmonic acid carboxyl methyltransferase gene (AtJMT) linked to the Ubi1 promoter increased levels of methyl jasmonate (MeJA) by 6-fold in young panicles. Grain yield was greatly reduced in Ubi1: AtJMT plants due to a lower numbers of spikelets and lower filling rates than were observed for nontransgenic (NT) controls. Ubi1: AtJMT plants had altered numbers of spikelet organs, including the lemma/palea, lodicule, anther, and pistil. The loss of grain yield and alteration in spikelet organ numbers were reproduced by treating NT plants with exogenous MeJA, indicating that increased levels of MeJA in Ubi1: AtJMT panicles inhibited spikelet development. Interestingly, MeJA levels were increased by 19-fold in young NT panicles upon exposure to drought conditions, resulting in a loss of grain yield that was similar to that observed in Ubi1: AtJMT plants. Levels of abscisic acid (ABA) were increased by 1.9-and 1.4-fold in Ubi1: AtJMT and drought-treated NT panicles, respectively. The ABA increase in Ubi1: AtJMT panicles grown in nondrought conditions suggests that MeJA, rather than drought stress, induces ABA biosynthesis under drought conditions. Using microarray and quantitative polymerase chain reaction analyses, we identified seven genes that were regulated in both Ubi1: AtJMT and drought-treated NT panicles. Two genes, OsJMT1 and OsSDR (for short-chain alcohol dehydrogenase), are involved in MeJA and ABA biosynthesis, respectively, in rice (Oryza sativa). Overall, our results suggest that plants produce MeJA during drought stress, which in turn stimulates the production of ABA, together leading to a loss of grain yield.
引用
收藏
页码:1751 / 1760
页数:10
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