Mini-scale genomic DNA extraction from cotton

被引：45

作者：

Chaudhry, B ^{[1
]}

Yasmin, A ^{[1
]}

Husnain, T ^{[1
]}

Riazuddin, S ^{[1
]}

机构：

[1] Punjab Univ, Natl Ctr Excellence Mol Biol, Lahore 53700, Pakistan

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1999年 / 17卷 / 03期

关键词：

genomic DNA; gossypium; hot/dry environment; phenolics; transgenics;

D O I：

10.1023/A:1007629715971

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Large amounts of polyphenolics in cotton leaves make it difficult to obtain high-quality genomic DNA during extraction. A procedure to isolate nuclear DNA from local cotton leaves (gossypium hirsutum, MNH93, CIM443, FH672) was therefore developed. It consists of rapid isolation of stable nuclei, which hinders covalent interactions with phenolics, followed by DNA extraction. The yield and quality of the resulting DNA is satisfactory and the protocol can be scaled up or down according to sample size. It is suitable for PCR and the restriction enzyme digestion needed for Southern and RFLP analysis.

引用

页码：280 / 280

页数：1

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