Detection of Campylobacter jejuni and Camp coli in chicken faecal samples by PCR

被引：34

作者：

Rasmussen, HN

Olsen, JE

Jorgensen, K

Rasmussen, OF

机构：

[1] RVAU, CTR FOOD RES, INST BIOTECHNOL, LYNGBY, DENMARK

[2] ROYAL VET & AGR UNIV, DEPT VET MICROBIOL, COPENHAGEN, DENMARK

[3] RADIOMETER MED AS, COPENHAGEN, DENMARK

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1996年 / 23卷 / 05期

关键词：

D O I：

10.1111/j.1472-765X.1996.tb00209.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

PCR primers were selected from the flagellin gene sequences flaA and flaB of Campylobacter coli to amplify DNA from Camp. jejuni and Camp. coli. When the PCR products were analysed by hybridization to an internal probe immobilized in microtitre wells, positive reactions were observed only for strains of Camp. jejuni and Camp. coli. The assay was used to analyse 31 chicken faecal samples. Full correspondence was found between the PCR assay conducted on the enriched cultures and the standard culture method. When analysing the transport medium prior to enrichment, the PCR assay detected nine of 11 culture positive samples.

引用

页码：363 / 366

页数：4

共 24 条

[1]

AHO M, 1988, ACTA VET SCAND, V29, P451

[2] DETECTION OF HIV-1 DNA BY A SIMPLE PROCEDURE OF POLYMERASE CHAIN-REACTION, USING PRIMER-DIMER FORMATION AS AN INTERNAL CONTROL OF AMPLIFICATION [J].