Near real time confocal microscopy of amelanotic tissue: Dynamics of aceto-whitening enable nuclear segmentation

被引:56
作者
Collier, T [1 ]
Shen, P
de Pradier, B
Sung, KB
Richards-Kortum, R
Malpica, A
Follen, M
机构
[1] Univ Texas, Dept Elect & Comp Engn, Austin, TX 78712 USA
[2] Univ Texas, MD Anderson Canc Ctr, Dept Pathol, Houston, TX 78730 USA
[3] Univ Texas, MD Anderson Canc Ctr, Dept Gynecol Oncol, Houston, TX 78730 USA
来源
OPTICS EXPRESS | 2000年 / 6卷 / 02期
关键词
D O I
10.1364/OE.6.000040
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
High resolution, in vivo confocal imaging of amelanotic epithelial tissue may offer a clinically useful adjunct to standard histopathologic techniques. Application of acetic acid has been shown to enhance contrast in confocal images of these tissues. In this study, we record the time course of aceto-whitening at the cellular level and determine whether the contrast provided enables quantitative feature analysis. Confocal images and videos of cervical specimens were obtained throughout the epithelium before, during and post-acetic acid after the application of 6% acetic acid. Aceto-whitening occurs within seconds after the application. The confocal imaging system resolved sub-cellular detail throughout the entire epithelial thickness and provided sufficient contrast to enable quantitative feature analysis. (C) 2000 Optical Society of America.
引用
收藏
页码:40 / 48
页数:9
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