Sequence-specific binding of single-stranded RNA: is there a code for recognition?

被引：242

作者：

Auweter, Sigrid D.

Oberstrass, Florian C.

Allain, Frederic H. -T. ^{[1
]}

机构：

[1] ETH, Inst Mol Biol & Biophys, Dept Biol, CH-8093 Zurich, Switzerland

[2] Mol Life Sci PhD Program, Zurich, Switzerland

来源：

NUCLEIC ACIDS RESEARCH | 2006年 / 34卷 / 17期

关键词：

D O I：

10.1093/nar/gkl620

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A code predicting the RNA sequence that will be bound by a certain protein based on its amino acid sequence or its structure would provide a useful tool for the design of RNA binders with desired sequence-specificity. Such de novo designed RNA binders could be of extraordinary use in both medical and basic research applications. Furthermore, a code could help to predict the cellular functions of RNA-binding proteins that have not yet been extensively studied. A comparative analysis of Pumilio homology domains, zinc-containing RNA binders, hnRNP K homology domains and RNA recognition motifs is performed in this review. Based on this, a set of binding rules is proposed that hints towards a code for RNA recognition by these domains. Furthermore, we discuss the intermolecular interactions that are important for RNA binding and summarize their importance in providing affinity and specificity.

引用

收藏

页码：4943 / 4959

页数：17

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