Photodynamic triggering of calcium oscillation in the isolated rat pancreatic acini

被引：55

作者：

Cui, ZJ ^{[1
]}

Kanno, T ^{[1
]}

机构：

[1] HOKKAIDO UNIV,GRAD SCH VET MED,DEPT BIOMED SCI,SAPPORO,HOKKAIDO 060,JAPAN

来源：

JOURNAL OF PHYSIOLOGY-LONDON | 1997年 / 504卷 / 01期

关键词：

D O I：

10.1111/j.1469-7793.1997.047bf.x

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

1. Photodynamic agents, due to their photon-dependent selective activation, can selectively activate a number of physiological processes and may directly modulate signal transduction in a number of cells including pancreatic acinar cells. 2. Activation of the photodynamic agent sulphonated aluminium phthalocyanine (SALPC) triggered recurrent cytosolic calcium ([Ca2+](i)) spiking in pancreatic acinar cells. 3. The photodynamically triggered calcium spiking could be blocked by phosphatidylinositol-specific phospholipase C (PI-PLC) inhibitor U73122, but not by phosphatidylcholine-specific phospholipase C inhibitor D809. 4. Removal of extracellular Ca2+ abolished spiking, as did 2-aminoethoxydiphenylborate (2-APB), an inhibitory modulator of IP3-mediated Ca2+ release from intracellular stores. 5. These data suggest that SALPC photodynamic action may permanently fix PI-PLC in an active conformation, and this produced recurrent [Ca2+](i) spiking.

引用

页码：47 / 55

页数：9

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