Generation of Cre recombinase-specific monoclonal antibodies, able to characterize the pattern of Cre expression in cre-transgenic mouse strains

被引:22
作者
Schwenk, F
Sauer, B
Kukoc, N
Hoess, R
Muller, W
Kocks, C
Kuhn, R
Rajewsky, K
机构
[1] NIDDK, NIH, BETHESDA, MD 20892 USA
[2] DUPONT CO INC, GENET & CANC GRP, WILMINGTON, DE 19880 USA
关键词
conditional gene targeting; Cre recombinase; monoclonal antibodies;
D O I
10.1016/S0022-1759(97)00116-6
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Transgene-encoded Cre recombinase can target alteration of loxP-tagged genes to specific cell types and developmental stages in mice, depending on the pattern of transgene expression. To facilitate determination of the latter, we have generated monoclonal anti-Cre antibodies which are specific for distinct epitopes on the recombinase and detect Cre both on immunoblots and intracellularly by immunofluorescence. We demonstrate the usefulness of these antibodies by an analysis of Cre expression in mice carrying a cre-transgene under B cell-specific control. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:203 / 212
页数:10
相关论文
共 36 条
[1]   STUDIES ON THE PROPERTIES OF P1 SITE-SPECIFIC RECOMBINATION - EVIDENCE FOR TOPOLOGICALLY UNLINKED PRODUCTS FOLLOWING RECOMBINATION [J].
ABREMSKI, K ;
HOESS, R ;
STERNBERG, N .
CELL, 1983, 32 (04) :1301-1311
[2]  
[Anonymous], 1988, Antibodies: A Laboratory Manual
[3]   FLOW CYTOMETRIC DETERMINATION OF CYTOKINES IN ACTIVATED MURINE T-HELPER LYMPHOCYTES - EXPRESSION OF INTERLEUKIN-10 IN INTERFERON-GAMMA AND IN INTERLEUKIN-4-EXPRESSING CELLS [J].
ASSENMACHER, M ;
SCHMITZ, J ;
RADBRUCH, A .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1994, 24 (05) :1097-1101
[4]  
Coffman R L, 1982, Immunol Rev, V69, P5
[5]   IMMUNOGLOBULIN HEAVY AND LIGHT CHAIN GENES REARRANGE INDEPENDENTLY AT EARLY STAGES OF B-CELL DEVELOPMENT [J].
EHLICH, A ;
SCHAAL, S ;
GU, H ;
KITAMURA, D ;
MULLER, W ;
RAJEWSKY, K .
CELL, 1993, 72 (05) :695-704
[6]  
Forster I, 1989, Int Immunol, V1, P321, DOI 10.1093/intimm/1.4.321
[7]   DELETION OF A DNA-POLYMERASE-BETA GENE SEGMENT IN T-CELLS USING CELL-TYPE-SPECIFIC GENE TARGETING [J].
GU, H ;
MARTH, JD ;
ORBAN, PC ;
MOSSMANN, H ;
RAJEWSKY, K .
SCIENCE, 1994, 265 (5168) :103-106
[8]   INDEPENDENT CONTROL OF IMMUNOGLOBULIN SWITCH RECOMBINATION AT INDIVIDUAL SWITCH REGIONS EVIDENCED THROUGH CRE-IOXP-MEDIATED GENE TARGETING [J].
GU, H ;
ZOU, YR ;
RAJEWSKY, K .
CELL, 1993, 73 (06) :1155-1164
[9]   T-LYMPHOCYTE TISSUE-CULTURE LINES PRODUCED BY CELL HYBRIDIZATION [J].
HAMMERLING, GJ .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1977, 7 (10) :743-746
[10]   RESOLUTION AND CHARACTERIZATION OF PRO-B AND PRE-PRO-B CELL STAGES IN NORMAL MOUSE BONE-MARROW [J].
HARDY, RR ;
CARMACK, CE ;
SHINTON, SA ;
KEMP, JD ;
HAYAKAWA, K .
JOURNAL OF EXPERIMENTAL MEDICINE, 1991, 173 (05) :1213-1225