Temporal and spatial delineation of mouse Otx2 functions by conditional self-knockout

被引：49

作者：

Fossat, Nicolas ^{[1
]}

Chatelain, Gilles ^{[1
]}

Brun, Gilbert ^{[1
]}

Lamonerie, Thomas ^{[1
]}

机构：

[1] CNRS, IFR128, INRA, ENS 1237,LBMC,UMR 5161, Lyon, France

来源：

EMBO REPORTS | 2006年 / 7卷 / 08期

关键词：

CreER(T2); tamoxifen; development; brain; Otx2;

D O I：

10.1038/sj.embor.7400751

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To identify the independent spatial and temporal activities of the essential developmental gene Otx2, the germline mutation of which is lethal at embryonic day 8.5, we floxed one allele and substituted the other with an inducible CreER recombinase gene. This makes 'trans' self-knockout possible at any developmental stage. The transient action of tamoxifen pulses allows time-course mutation. We demonstrate efficient temporal knockout and demarcate spatio-temporal windows in which Otx2 controls the head, brain structures and body development.

引用

页码：824 / 830

页数：7

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