The absence of D-alanine from lipoteichoic acid and wall teichoic acid alters surface charge, enhances autolysis and increases susceptibility to methicillin in Bacillus subtilis

被引:56
作者
Wecke, J [1 ]
Madela, K [1 ]
Fischer, W [1 ]
机构
[1] UNIV ERLANGEN NURNBERG,INST BIOCHEM,D-91054 ERLANGEN,GERMANY
来源
MICROBIOLOGY-UK | 1997年 / 143卷
关键词
Bacillus subtilis; lipoteichoic acid; wall teichoic acid; autolysis; methicillin;
D O I
10.1099/00221287-143-9-2953
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In Bacillus subtilis the physiological consequences of depriving lipoteichoic acid and wall teichoic acid of D-alanine ester were analysed using insertional inactivation of the genes of the dlt operon. Mutant strains which lacked positively charged D-alanine ester in teichoic acids bound more positively charged cytochrome c than other strains. These mutant strains also showed enhanced autolysis and a higher susceptibility to methicillin, which was expressed as accelerated wall lysis, a faster loss of viability and a slower recovery in the postantibiotic phase. The effects of methicillin could be suppressed by simultaneous addition of magnesium ions at low concentrations. The degradation of whole bacteria by bone-marrow-derived macrophages was not influenced by the surface charge and alanylation of the teichoic acids had no protective effect.
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页码:2953 / 2960
页数:8
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