Combined dielectrophoretic field cages and laser tweezers for electrorotation

被引:41
作者
Schnelle, T [1 ]
Müller, T [1 ]
Reichle, C [1 ]
Fuhr, G [1 ]
机构
[1] Humboldt Univ, Inst Biol, D-10115 Berlin, Germany
来源
APPLIED PHYSICS B-LASERS AND OPTICS | 2000年 / 70卷 / 02期
关键词
D O I
10.1007/s003400050044
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Symmetric rotating octode field cages do not allow simultaneous particle trapping and electrorotation. Laser-trapped particles can stably be held in inhomogeneous rotating electric fields. Therefore, in a combination of laser tweezers and field-cage (electro-optical cage) dielectric single-particle spectroscopy (DSPS) of particles and cells can be carried out at low electric field strength. The electro-optical cage overcomes the limitation of low enough effective particle conductivity ensuring repelling dielectrophoretic forces in conventional field cages. At low power, forces in field cages and laser tweezers hardly interfere and can be used to calibrate each other.
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页码:267 / 274
页数:8
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