Towards many colors in FISH on 3D-preserved interphase nuclei

被引：38

作者：

Walter, J.

Joffe, B.

Bolzer, A.

Albiez, H.

Benecletti, P. A.

Mueller, S.

Speicher, M. R.

Cremer, T.

Cremer, M.

Solovei, I.

机构：

[1] Univ Munich, Biozentrum, Dept Biol 2, Chair Anthropol & Human Genet, DE-82152 Martinsried, Germany

[2] Univ Munich, BioImaging Zentrum, TILL ID GmbH, DE-82152 Martinsried, Germany

[3] CNR, IPCF, I-56100 Pisa, Italy

[4] Tech Univ Munich, D-8000 Munich, Germany

[5] GSF Munich, Inst Human Genet, Munich, Germany

来源：

CYTOGENETIC AND GENOME RESEARCH | 2006年 / 114卷 / 3-4期

关键词：

D O I：

10.1159/000094227

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The article reviews the existing methods of multicolor FISH on nuclear targets, first of all, interphase chromosomes. FISH proper and image acquisition are considered as two related components of a single process. We discuss (1) M-FISH (combinatorial labeling + deconvolution + widefield microscopy); (2) multicolor labeling + SIM (structured illumination microscopy); (3) the standard approach to multicolor FISH + CLSM (confocal laser scanning microscopy; one fluorochrome - one color channel); (4) combinatorial labeling + CLSM; (5) non-combinatorial labeling + CLSM + linear unmixing. Two related issues, deconvolution of images acquired with CLSM and correction of data for chromatic Z-shift, are also discussed. All methods are illustrated with practical examples. Finally, several rules of thumb helping to choose an optimal labeling + microscopy combination for the planned experiment are suggested. Copyright (c) 2006 S. Karger AG, Basel.

引用

页码：367 / 378

页数：12

共 31 条

[1] An optimized probe set for the detection of small interchromosomal aberrations by use of 24-color FISH [J].