Isolation and characterization of microsatellites in Pacific white shrimp Penaeus (Litopenaeus) vannamei

被引：24

作者：

Cruz, P ^{[1
]}

Mejia-Ruiz, CH ^{[1
]}

Perez-Enriquez, R ^{[1
]}

Ibarra, AM ^{[1
]}

机构：

[1] CIBNOR SC, Ctr Invest Biol Noroeste, La Paz BCS, Mexico

来源：

MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 03期

关键词：

breeding programs; microsatellites; Pacific white shrimp; Penaeus vannamei; population genetics;

D O I：

10.1046/j.1471-8286.2002.00211.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Five polymorphic microsatellite loci were characterized for Penaeus (Litopenaeus) vannamei. Loci were isolated using a partial Sau3A1 genomic library by the sequencing of randomly selected clones and by a biotinylated (CT)(10) and (GT)(10) probes screening procedure. The last strategy resulted in the most useful data. About 40% of the clones showed a previously reported satellite/microsatellite (PVS1), reducing the chance of finding new microsatellite regions. Whereas two of the microsatellite loci with more than 10 alleles will be useful for mating analysis in a breeding program, the others might prove useful for population genetic studies.

引用

页码：239 / 241

页数：3

共 11 条

[1] A novel satellite/microsatellite combination in the genome of the marine shrimp, Penaeus vannamei [J].

Bagshaw, JC ;

Buckholt, MA .

GENE, 1997, 184 (02) :211-214

[2]

Ball AO, 1998, MOL ECOL, V7, P1251

[3] Population genetic structure in penaeid prawns [J].

Benzie, JAH .

AQUACULTURE RESEARCH, 2000, 31 (01) :95-119

[4]

Garcia DK, 1996, MOL MAR BIOL BIOTECH, V5, P71

[5]

MCCARTY C, 1998, CHROMAS 1 45 FREE PR

[6] Genetic variability and pedigree tracing of a hatchery-reared stock of red sea bream (Pagrus major) used for stock enhancement, based on microsatellite DNA markers [J].

Perez-Enriquez, R ;

Takagi, M ;

Taniguchi, N .

AQUACULTURE, 1999, 173 (1-4) :413-423

[7]

ROZEN S, 1997, PRIMER 3

[8]

Sambrook J., 1989, MOL CLONING

[9]

*SEMARNAP, 1998, AN EST PESC

[10] Application of the microsatellite technique for analyzing genetic diversity in shrimp breeding programs [J].

Wolfus, GM ;

Garcia, DK ;

AlcivarWarren, A .

AQUACULTURE, 1997, 152 (1-4) :35-47

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