Double strand break rejoining after irradiation of human fibroblasts with X rays or α particles:: PFGE studies and numerical models

被引:18
作者
Pinto, M [1 ]
Prise, KM [1 ]
Michael, BD [1 ]
机构
[1] Mt Vernon Hosp, Gray Canc Inst, Northwood HA6 2JR, Middx, England
关键词
D O I
10.1093/oxfordjournals.rpd.a006743
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
When a charged-particle track intercepts the chromatin fibre in DNA of mammalian cells, clustered damage is induced depending on the DNA conformation, local environment and track structure. Intra-track correlated DNA damage may have a higher probability of being mis-repaired or left un-repaired. Fragment size-distributions of DNA double strand breaks (DSBs) induced in primary human fibroblasts by 240kV(p) X rays and Pu-238 alpha particles (110keV.mum (-1)) were resolved using pulsed-field gel electrophoresis (PFGE). By monitoring DSB rejoining kinetics and changes in the fragment size distribution with repair time, the relevance of spatial association of DSB, in determining rejoining kinetics was investigated. Rejoining kinetics appeared bi-phasic and independent of the size of the DNA fragments for both radiation qualities, with high LET radiation-induced DSBs repairing more slowly. Results suggest that local coplexity of individual DSBs, rather than spatial association with other breaks is more significant in the determination of rejoining kinetics.
引用
收藏
页码:133 / 136
页数:4
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