Bacterial community composition and chitinase gene diversity of vermicompost with antifungal activity

被引：112

作者：

Yasir, Muhammad ^{[1
,2
]}

Aslam, Zubair ^{[1
,2
]}

Kim, Seon Won ^{[1
,2
]}

Lee, Seon-Woo ^{[3
]}

Jeon, Che Ok ^{[4
]}

Chung, Young Ryun ^{[1
,2
]}

机构：

[1] Gyeongsang Natl Univ, Div Appl Life Sci BK 21, PMBBRC, Jinju 660701, South Korea

[2] Gyeongsang Natl Univ, EB NCRC, Jinju 660701, South Korea

[3] Dong A Univ, Coll Nat Resources & Life Sci, Dept Appl Biol, Pusan 604714, South Korea

[4] Chung Ang Univ, Dept Life Sci, Seoul 156756, South Korea

来源：

BIORESOURCE TECHNOLOGY | 2009年 / 100卷 / 19期

关键词：

Bacterial community; Chitinase; Plant pathogenic fungi; Paper sludge; Vermicomposting; STREPTOMYCES-GRISEUS; MICROBIAL BIOMASS; EISENIA-FOETIDA; EARTHWORM GUTS; PAPER-MILL; CULTURE; QUALITY; SLUDGE; SOIL;

D O I：

10.1016/j.biortech.2009.04.015

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Bacterial communities and chitinase gene diversity of vermicompost (VC) were investigated to clarify the influence of earthworms on the inhibition of plant pathogenic fungi in VC. The spore germination of Fusarium moniliforme was reduced in VC aqueous extracts prepared from paper sludge and dairy sludge (fresh sludge, FS). The bacterial communities were examined by culture-dependent and -independent analyses. Unique clones selected from 16S rRNA libraries of FS and VC on the basis of restriction fragment length polymorphism (RFLP) fell into the major lineages of the domain bacteria Proteobacteria, Bacteroidetes, Verrucomicrobia, Actinobacteria and Firmicutes. Among culture isolates, Actinobacteria dominated in VC, while almost equal numbers of Actinobacteria and Proteobacteria were present in FS. Analysis of chitinolytic isolates and chitinase gene diversity revealed that chitinolytic bacterial communities were enriched in VC. Populations of bacteria that inhibited plant fungal pathogens were higher in VC than in FS and particularly chitinolytic isolates were most active against the target fungi. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：4396 / 4403

页数：8

共 35 条

[1] Earthworms strongly modify microbial biomass and activity triggering enzymatic activities during vermicomposting independently of the application rates of pig slurry [J].