Real-Time Quantitative RT-PCR: Design, Calculations, and Statistics

被引：364

作者：

Rieu, Ivo ^{[1
]}

Powers, Stephen J. ^{[2
]}

机构：

[1] Univ Freiburg, Inst Biol 3, Lab Mol Genet & Biotechnol Plants, D-79104 Freiburg, Germany

[2] Ctr Math & Computat Biol, Biomath & Bioinformat Dept, Harpenden AL5 2JQ, Herts, England

来源：

PLANT CELL | 2009年 / 21卷 / 04期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1105/tpc.109.066001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：1031 / 1033

页数：3

共 13 条

[1] Model based analysis of real-time PCR data from DNA binding dye protocols [J].

Alvarez, Mariano J. ;

Vila-Ortiz, Guillermo J. ;

Salibe, Mariano C. ;

Podhajcer, Osvaldo L. ;

Pitossi, Fernando J. .

BMC BIOINFORMATICS, 2007, 8 (1)

[2] SAS programs for real-time RT PCR having multiple independent samples [J].

Cook, P ;

Fu, CX ;

Hickey, M ;

Han, ES ;

Miller, KS .

BIOTECHNIQUES, 2004, 37 (06) :990-995

[3] Evaluation of quantification methods for real-time PCR minor groove binding hybridization probe assays [J].

Durtschi, Jacob D. ;

Stevenson, Jeffery ;

Hymas, Weston ;

Voelkerding, Karl V. .

ANALYTICAL BIOCHEMISTRY, 2007, 361 (01) :55-64

[4]

Gomez KA., 1984, STAT PROCEDURES AGR

[5] Towards a systematic validation of references in real-time RT-PCR [J].

Gutierrez, Laurent ;

Mauriat, Melanie ;

Pelloux, Jerome ;

Bellini, Catherine ;

van Wuytswinkel, Olivier .

PLANT CELL, 2008, 20 (07) :1734-1735

[6] qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data [J].

Hellemans, Jan ;

Mortier, Geert ;

De Paepe, Anne ;

Speleman, Frank ;

Vandesompele, Jo .

GENOME BIOLOGY, 2007, 8 (02)

[7]

Mead Roger., 1988, The Design of Experiments: Statistical Principles for Practical Applicati

[8] RECOVERY OF INTER-BLOCK INFORMATION WHEN BLOCK SIZES ARE UNEQUAL [J].

PATTERSON, HD ;

THOMPSON, R .

BIOMETRIKA, 1971, 58 (03) :545-+

[9]

Pfaffl Michael W., 2009, P65

[10] Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data [J].

Ramakers, C ;

Ruijter, JM ;

Deprez, RHL ;

Moorman, AFM .

NEUROSCIENCE LETTERS, 2003, 339 (01) :62-66

← 1 2 →