Lanthanum ions inhibit the mammalian Sec61 complex in its channel dynamics and protein transport activity

被引：18

作者：

Erdmann, Frank ^{[1
]}

Jung, Martin ^{[2
]}

Eyrisch, Susanne ^{[3
]}

Lang, Sven ^{[2
]}

Helms, Volkhard ^{[3
]}

Wagner, Richard ^{[1
]}

Zimmermann, Richard ^{[2
]}

机构：

[1] Univ Osnabruck, FB Biol Chem, D-49034 Osnabruck, Germany

[2] Univ Saarland, D-66421 Homburg, Germany

[3] Univ Saarland, D-66041 Saarbrucken, Germany

来源：

FEBS LETTERS | 2009年 / 583卷 / 14期

关键词：

Electrophysiological property; Endoplasmic reticulum; Lanthanum; Protein transport; Sec61; complex; CONDUCTING CHANNEL; TRANSLOCATION; ARCHITECTURE; COMPONENTS; MEMBRANE;

D O I：

10.1016/j.febslet.2009.06.032

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous electrophysiological experiments characterized the Sec61 complex, which provides the aqueous path for entry of newly-synthesized polypeptides into the mammalian endoplasmic reticulum, as a highly dynamic channel that, once activated by precursor proteins, fluctuates between main open states with mean conductances of 220 and 550 pS. Millimolar concentrations of lanthanum ions simultaneously restricted the dynamics of the Sec61 channel and inhibited translocation of polypeptides. Molecular modeling indicates that lanthanum binding sites cluster at the putative lateral gate of the Sec61 complex and suggests that structural flexibility of the lateral gate is essential for channel and protein transport activities of the Sec61 complex. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2359 / 2364

页数：6

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