Factors affecting the biodegradation of cyanide by Burkholderia cepacia strain C-3

被引:43
作者
Adjei, MD [1 ]
Ohta, Y [1 ]
机构
[1] Hiroshima Univ, Fac Appl Biol Sci, Microbial Biochem Lab, Higashihiroshima 7398528, Japan
关键词
cyanide biodegradation; cyanide-utilizing bacteria; Burkholderia cepacia;
D O I
10.1016/S1389-1723(00)88833-7
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The utilization of cyanide as a nitrogen source for growth in a liquid minimal medium by Burkholderia cepacia strain C-3 isolated from soil was demonstrated. The effects of various parameters on the biodegradation of cyanide by the strain were investigated. Growth at the expense of cyanide occurred over a pH range from 8 to 10, and was optimal at pH 10. Growth and cyanide biodegradation were optimal at 30 degrees C. The presence of Cu2+ or Fe2+ in the liquid medium at a concentration of 1 mM inhibited both the growth of the bacterium and its cyanide degradation. The effects of Ni2+ and Co2+, and to a lesser extent Mn2+ and Mo2+, On cyanide degradation rate were concentration dependent. Addition of the cyanide-containing waste contaminants ethanol and methanol reduced the cyanide utilization of the isolate, but phenol was completely inhibitory. Microbial depletion of cyanide occurred even in the presence of other organic and inorganic nitrogen sources. Fructose, glucose, and mannose were the preferred carbon sources for cyanide biodegradation. The highest rate of cyanide degradation by B. cepacia C-3, 1.85 mg CN h(-1), was observed with 0.25% (w/v) fructose; the degradation rate decreased progressively thereafter with increasing fructose concentration.
引用
收藏
页码:274 / 277
页数:4
相关论文
共 18 条
[1]   Isolation and characterization of a cyanide-utilizing Burkholderia cepacia strain [J].
Adjei, MD ;
Ohta, Y .
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1999, 15 (06) :699-704
[2]   Cyanide degradation under alkaline conditions by a strain of Fusarium solani isolated from contaminated soils [J].
Dumestre, A ;
Chone, T ;
Portal, JM ;
Gerard, M ;
Berthelin, J .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (07) :2729-2734
[3]   IDENTIFICATION AND CHARACTERIZATION OF AN ACINETOBACTER SP CAPABLE OF ASSIMILATION OF A RANGE OF CYANO-METAL COMPLEXES, FREE CYANIDE IONS AND SIMPLE ORGANIC NITRILES [J].
FINNEGAN, I ;
TOERIEN, S ;
ABBOT, L ;
SMIT, F ;
RAUBENHEIMER, HG .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1991, 36 (01) :142-144
[4]   COLORIMETRIC DETERMINATION OF CYANIDE IN STACK GAS AND WASTE WATER [J].
FISHER, FB ;
BROWN, JS .
ANALYTICAL CHEMISTRY, 1952, 24 (09) :1440-1444
[5]  
HARRIS R, 1983, J GEN MICROBIOL, V129, P1005
[7]  
HOPE KM, 1991, FEMS MICROBIOL LETT, V80, P217, DOI 10.1111/j.1574-6968.1991.tb04664.x
[8]   NOVEL CYANIDE-HYDROLYZING ENZYME FROM ALCALIGENES-XYLOSOXIDANS SUBSP DENITRIFICANS [J].
INGVORSEN, K ;
HOJERPEDERSEN, B ;
GODTFREDSEN, SE .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (06) :1783-1789
[9]   DEGRADATION OF CYANIDE BY A BACTERIAL MIXTURE COMPOSED OF NEW TYPES OF CYANIDE-DEGRADING BACTERIA [J].
KANG, SM ;
KIM, DJ .
BIOTECHNOLOGY LETTERS, 1993, 15 (02) :201-206
[10]   MICROORGANISMS AND CYANIDE [J].
KNOWLES, CJ .
BACTERIOLOGICAL REVIEWS, 1976, 40 (03) :652-680