Increased DNA binding and sequence discrimination of PNA oligomers containing 2,6-diaminopurine

被引:96
作者
Haaima, G
Hansen, HF
Christensen, L
Dahl, O
Nielsen, PE
机构
[1] UNIV COPENHAGEN, PANUM INST, DEPT BIOCHEM & GENET, LAB B, DK-2200 COPENHAGEN N, DENMARK
[2] HC ORSTED INST, DEPT CHEM, CTR BIOMOL RECOGNIT, DK-2100 COPENHAGEN O, DENMARK
关键词
D O I
10.1093/nar/25.22.4639
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The synthesis of a diaminopurine PNA monomer, N[N-6-(benzyloxycarbonyl)-2,6-diaminopurine-9-yl] acetyl-N-(2-t-butyloxycarbonylaminoethyl)glycine, and the incorporation of this monomer into PNA oligomers am described, Substitution of adenine by diaminopurine in PNA oligomers increased the T-m of duplexes formed with complementary DNA, RNA or PNA by 2.5-6.5 degrees C per diaminopurine, Furthermore, discrimination against mismatches facing the diaminopurine in the hybridizing oligomer is improved, Finally, a homopurine decamer PNA containing six diaminopurines is shown to form a (gel shift) stable strand displacement complex with a target in a 246 bp double-stranded DNA fragment.
引用
收藏
页码:4639 / 4643
页数:5
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